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bacteria:t3e:xopbh [2025/05/07 09:42] – [Biological function] rkoebnikbacteria:t3e:xopbh [2025/07/24 14:11] (current) – [The Type III Effector XopBH from //Xanthomonas//] rkoebnik
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 Author: [[https://www.researchgate.net/profile/Naama-Wagner|Naama Wagner]]\\ Author: [[https://www.researchgate.net/profile/Naama-Wagner|Naama Wagner]]\\
-Internal reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Internal reviewer: [[https://www.researchgate.net/profile/Leonor-Martins-2|Leonor Martins]]\\ 
 +Expert reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]
  
 Class: XopBH\\ Class: XopBH\\
 Family: XopBH\\ Family: XopBH\\
-Prototype: XTG_RS02340 (//Xanthomonas euroxanthea//, strain CPBF 424) Attention: The prototype sequence is too long because codons 18 to 39 overlap with the plant-inducible promoter (see below; Koebnik //et al.//, 2006).\\+Prototype: XTG_RS02340 (//Xanthomonas euroxanthea//, strain CPBF 424). Attention: The prototype sequence is too long because codons 18 to 39 overlap with the plant-inducible promoter (see below; Koebnik //et al.//, 2006).\\
 GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/CAE1133144.1|CAJ22212.1]] (286 aa)\\ GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/CAE1133144.1|CAJ22212.1]] (286 aa)\\
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_212580660.1|WP_039417318.1]] (216 aa)\\ RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_212580660.1|WP_039417318.1]] (216 aa)\\
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 XopBH was discovered by a [[https://effectidor.tau.ac.il|Effectidor II]], a pan-genomic AI-based algorithm for the prediction of type III secretion system effectors (Wagner //et al.//, 2025). The //xopBH// gene of strain CPBF 424 is located near the T3SS gene cluster, next to [[:bacteria:t3e:xopz|xopZ]] and [[:bacteria:t3e:xopf|xopF]], which are all encoded between //hrpE// and //hrpF// (Huguet & Bonas, 1997; Weber //et al.//, 2005). XopBH was discovered by a [[https://effectidor.tau.ac.il|Effectidor II]], a pan-genomic AI-based algorithm for the prediction of type III secretion system effectors (Wagner //et al.//, 2025). The //xopBH// gene of strain CPBF 424 is located near the T3SS gene cluster, next to [[:bacteria:t3e:xopz|xopZ]] and [[:bacteria:t3e:xopf|xopF]], which are all encoded between //hrpE// and //hrpF// (Huguet & Bonas, 1997; Weber //et al.//, 2005).
 +
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 XopBH was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013). XopBH was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 +
 === Regulation === === Regulation ===
  
 The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopBH// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006). The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopBH// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006).
 +
 === Phenotypes === === Phenotypes ===
  
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 Yes (//e.g.//, //X. arboricola//, //X. campestris// pv. //papavericola//, //X. hortorum//). Yes (//e.g.//, //X. arboricola//, //X. campestris// pv. //papavericola//, //X. hortorum//).
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
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 ===== References ===== ===== References =====
 +
 +Huguet E, Bonas U (1997). //hrpF// of //Xanthomonas campestris// pv. //vesicatoria// encodes an 87-kDa protein with homology to NoIX of //Rhizobium fredii//. Mo.l Plant Microbe Interact. 10: 488-498. DOI: [[https://doi.org/10.1094/MPMI.1997.10.4.488|10.1094/MPMI.1997.10.4.488]]
  
 Koebnik R, Krüger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the //Xanthomonas campestris// pv. //vesicatoria// AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https://doi.org/10.1128/JB.00795-06|10.1128/JB.00795-06]] Koebnik R, Krüger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the //Xanthomonas campestris// pv. //vesicatoria// AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https://doi.org/10.1128/JB.00795-06|10.1128/JB.00795-06]]
  
 Wagner N, Baumer E, Lyubman I, Shimony Y, Bracha N, Martins L, Potnis N, Chang JH, Teper D, Koebnik R, Pupko T (2025). Effectidor II: A pan-genomic AI-based algorithm for the prediction of type III secretion system effectors. Bioinformatics, in press. DOI: [[https://doi.org/10.1093/bioinformatics/btaf272|10.1093/bioinformatics/btaf272]] Wagner N, Baumer E, Lyubman I, Shimony Y, Bracha N, Martins L, Potnis N, Chang JH, Teper D, Koebnik R, Pupko T (2025). Effectidor II: A pan-genomic AI-based algorithm for the prediction of type III secretion system effectors. Bioinformatics, in press. DOI: [[https://doi.org/10.1093/bioinformatics/btaf272|10.1093/bioinformatics/btaf272]]
 +
 +Weber E, Ojanen-Reuhs T, Huguet E, Hause G, Romantschuk M, Korhonen TK, Bonas U, Koebnik R (2005). The type III-dependent Hrp pilus is required for productive interaction of //Xanthomonas campestris// pv. //vesicatoria// with pepper host plants. J. Bacteriol. 187: 2458-2468. DOI: [[https://doi.org/10.1128/JB.187.7.2458-2468.2005|10.1128/JB.187.7.2458-2468.2005]]
  
 Wengelnik K, Bonas U (1996). HrpXv, an AraC-type regulator, activates expression of five of the six loci in the hrp cluster of //Xanthomonas campestris// pv. //vesicatoria//. J. Bacteriol. 178: 3462-3469. DOI: [[https://doi.org/10.1128/jb.178.12.3462-3469.1996|10.1128/jb.178.12.3462-3469.1996]] Wengelnik K, Bonas U (1996). HrpXv, an AraC-type regulator, activates expression of five of the six loci in the hrp cluster of //Xanthomonas campestris// pv. //vesicatoria//. J. Bacteriol. 178: 3462-3469. DOI: [[https://doi.org/10.1128/jb.178.12.3462-3469.1996|10.1128/jb.178.12.3462-3469.1996]]
bacteria/t3e/xopbh.1746607372.txt.gz · Last modified: 2025/05/07 09:42 by rkoebnik

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