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bacteria:t3e:xopbg

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bacteria:t3e:xopbg [2023/12/18 15:15] – [XopBG] rkoebnikbacteria:t3e:xopbg [2025/01/27 22:33] (current) – [Biological function] jfpothier
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-====== XopBG ======+====== The Type III Effector XopBG from //Xanthomonas// ======
  
-Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\ +Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]
-Expert reviewer: **WANTED!**+
  
 Class: XopBG\\ Class: XopBG\\
 Family: XopBG\\ Family: XopBG\\
-Prototype: BER92_RS22150 (//Xanthomonas fragariae//; strain Fap21) +Prototype: BER92_RS22150 (//Xanthomonas fragariae//; strain Fap21)\\
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_145953988.1|WP_145953988.1]] (158 aa)\\ RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_145953988.1|WP_145953988.1]] (158 aa)\\
-3D structure:+3D structure: Unknown
  
 ===== Biological function ===== ===== Biological function =====
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria //T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBG ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).+To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria// T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBG ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).
 === Regulation === === Regulation ===
  
bacteria/t3e/xopbg.1702912547.txt.gz · Last modified: 2023/12/18 15:15 by rkoebnik

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