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bacteria:t3e:xopbg [2023/12/18 13:43] – [XopBG] rkoebnikbacteria:t3e:xopbg [2025/07/04 23:26] (current) – jfpothier
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-====== XopBG ======+====== The Type III Effector XopBG from //Xanthomonas// ======
  
-Author: John Doe\\ +Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\
-Reviewer: Jane Doe+
  
 Class: XopBG\\ Class: XopBG\\
 Family: XopBG\\ Family: XopBG\\
-Prototype: BER92_RS22150 (//Xanthomonas fragariae//; strain Fap21) +Prototype: BER92_RS22150 (//Xanthomonas fragariae//; strain Fap21)\\ 
- +RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_145953988.1|WP_145953988.1]] (158 aa)\\ 
-RefSeq ID: WP_145953988.1\\ +3D structure: Unknown
-3D structure:+
  
 ===== Biological function ===== ===== Biological function =====
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 XopBG was discovered using a machine-learning approach for the prediction of T3Es (Wagner //et al.//, 2022, 2023). XopBG was discovered using a machine-learning approach for the prediction of T3Es (Wagner //et al.//, 2022, 2023).
 +
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria //T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs262–574 lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBG ORF, including 24 bp upstream of its ATG start codon, with AvrBs262–574 elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).+To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria// T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBG ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023). 
 === Regulation === === Regulation ===
  
 The //xopBG// gene contains a perfect PIP box in its promoter region and is likely co-regulated with the T3SS under control of HrpG and HrpX (Wagner //et al.//, 2023). The //xopBG// gene contains a perfect PIP box in its promoter region and is likely co-regulated with the T3SS under control of HrpG and HrpX (Wagner //et al.//, 2023).
 +
 === Phenotypes === === Phenotypes ===
  
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 Unique to //X. fragariae// (Wagner //et al.//, 2023). Unique to //X. fragariae// (Wagner //et al.//, 2023).
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
-No.+//Xylophilus ampelinus//.
  
 ===== References ===== ===== References =====
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 Wagner N, Ben-Meir D, Teper D, Pupko T (2023). Complete genome sequence of an Israeli isolate of //Xanthomonas hortorum// pv. //pelargonii// strain 305 and novel type III effectors identified in //Xanthomonas//. Front. Plant Sci. 14: 1155341. DOI: [[https://doi.org/10.3389/fpls.2023.1155341|10.3389/fpls.2023.1155341]] Wagner N, Ben-Meir D, Teper D, Pupko T (2023). Complete genome sequence of an Israeli isolate of //Xanthomonas hortorum// pv. //pelargonii// strain 305 and novel type III effectors identified in //Xanthomonas//. Front. Plant Sci. 14: 1155341. DOI: [[https://doi.org/10.3389/fpls.2023.1155341|10.3389/fpls.2023.1155341]]
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xopbg.1702906986.txt.gz · Last modified: 2023/12/18 13:43 by rkoebnik

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