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bacteria:t3e:xopbb [2025/01/27 22:31] – [Conservation] jfpothierbacteria:t3e:xopbb [2025/07/04 23:26] (current) – jfpothier
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 ====== The Type III Effector XopBB from //Xanthomonas// ====== ====== The Type III Effector XopBB from //Xanthomonas// ======
  
-Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]+Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\
  
 Class: XopBB\\ Class: XopBB\\
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 XopBB was discovered using a machine-learning approach for the prediction of T3Es (Wagner //et al.//, 2022, 2023). XopBB was discovered using a machine-learning approach for the prediction of T3Es (Wagner //et al.//, 2022, 2023).
 +
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria// T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBB ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023). To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria// T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBB ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).
 +
 === Regulation === === Regulation ===
  
 The //xopBB// gene contains a perfect PIP box in its promoter region and is likely co-regulated with the T3SS under control of HrpG and HrpX (Wagner //et al.//, 2023). The //xopBB// gene contains a perfect PIP box in its promoter region and is likely co-regulated with the T3SS under control of HrpG and HrpX (Wagner //et al.//, 2023).
 +
 === Phenotypes === === Phenotypes ===
  
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 Yes, //X. arboricola// and //X. hydrangeae// (Wagner //et al.//, 2023). Yes, //X. arboricola// and //X. hydrangeae// (Wagner //et al.//, 2023).
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
-Yes, in species of //Acidovorax // (//e.g.//, //A. avenae//, //A. citrulli//, //A. oryzae//) (Wagner //et al.//, 2023). XopBB is distantly related to HopF2 (syn. AvrPphF) from the //Pseudomonas syringae// species complex, e.g. //P. amygdali//, //P. cannabina// (ca. 30 % amino acid sequence identity).+Yes, in species of //Acidovorax// (//e.g.//, //A. avenae//, //A. citrulli//, //A. oryzae//) (Wagner //et al.//, 2023). XopBB is distantly related to HopF2 (syn. AvrPphF) from the //Pseudomonas syringae// species complex, e.g. //P. amygdali//, //P. cannabina// (ca. 30 % amino acid sequence identity).
  
 ===== References ===== ===== References =====
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 Wagner N, Ben-Meir D, Teper D, Pupko T (2023). Complete genome sequence of an Israeli isolate of //Xanthomonas hortorum// pv. //pelargonii// strain 305 and novel type III effectors identified in //Xanthomonas//. Front. Plant Sci. 14: 1155341. DOI: [[https://doi.org/10.3389/fpls.2023.1155341|10.3389/fpls.2023.1155341]] Wagner N, Ben-Meir D, Teper D, Pupko T (2023). Complete genome sequence of an Israeli isolate of //Xanthomonas hortorum// pv. //pelargonii// strain 305 and novel type III effectors identified in //Xanthomonas//. Front. Plant Sci. 14: 1155341. DOI: [[https://doi.org/10.3389/fpls.2023.1155341|10.3389/fpls.2023.1155341]]
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xopbb.1738017111.txt.gz · Last modified: 2025/01/27 22:31 by jfpothier

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