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bacteria:t3e:xopbb [2025/01/27 22:30] – [The Type III Effector XopBB from //Xanthomonas//] jfpothierbacteria:t3e:xopbb [2025/02/12 23:47] (current) – jfpothier
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria //T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBB ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).+To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria// T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBB ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).
 === Regulation === === Regulation ===
  
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 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
-Yes, in species of //Acidovorax // (e.g. //A. avenae//, //A. citrulli//, //A. oryzae//) (Wagner //et al.//, 2023). XopBB is distantly related to HopF2 (syn. AvrPphF) from the //Pseudomonas syringae// species complex, e.g. //P. amygdali//, //P. cannabina// (ca. 30 % amino acid sequence identity).+Yes, in species of //Acidovorax// (//e.g.//, //A. avenae//, //A. citrulli//, //A. oryzae//) (Wagner //et al.//, 2023). XopBB is distantly related to HopF2 (syn. AvrPphF) from the //Pseudomonas syringae// species complex, e.g. //P. amygdali//, //P. cannabina// (ca. 30 % amino acid sequence identity).
  
 ===== References ===== ===== References =====
bacteria/t3e/xopbb.1738017022.txt.gz · Last modified: 2025/01/27 22:30 by jfpothier

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