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--- bacteria:t3e:xopbb [2024/08/06 14:24] – [XopBB] rkoebnik
+++ bacteria:t3e:xopbb [2025/02/12 23:47] (current) – jfpothier
@@ Line 1: / Line 1: @@
-====== The Type III Effector XopBB from Xanthomonas ======
+====== The Type III Effector XopBB from //Xanthomonas// ======
-Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\
+Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]
-Expert reviewer: **WANTED!**
 Class: XopBB\\
 Family: XopBB\\
-Prototype: ELAGFFLI_02299 (//Xanthomonas ////hortorum// pv. //pelargonii//; strain 305)
+Prototype: ELAGFFLI_02299 (//Xanthomonas hortorum// pv. //pelargonii//; strain 305)\\
 GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/CAD0333498.1|CAD0333498]] (332 aa)\\
 RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_251761698.1|WP_251761698.1]] (220 aa, corresponding to the C-terminal domain)\\
-D structure:
+D structure: Unknown
 ===== Biological function =====
@@ Line 19: / Line 17: @@
 === (Experimental) evidence for being a T3E ===
-To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria //T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBB ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).
+To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria// T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBB ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).
 === Regulation ===
@@ Line 46: / Line 44: @@
 === In other plant pathogens/symbionts ===
-Yes, in species of //Acidovorax // (e.g. //A. avenae//, //A. citrulli//, //A. oryzae//) (Wagner //et al.//, 2023). XopBB is distantly related to HopF2 (syn. AvrPphF) from the //Pseudomonas syringae// species complex, e.g. //P. amygdali//, //P. cannabina// (ca. 30 % amino acid sequence identity).
+Yes, in species of //Acidovorax// (//e.g.//, //A. avenae//, //A. citrulli//, //A. oryzae//) (Wagner //et al.//, 2023). XopBB is distantly related to HopF2 (syn. AvrPphF) from the //Pseudomonas syringae// species complex, e.g. //P. amygdali//, //P. cannabina// (ca. 30 % amino acid sequence identity).
 ===== References =====

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