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bacteria:t3e:xopbb

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bacteria:t3e:xopbb [2023/12/18 14:37] – [XopBB] rkoebnikbacteria:t3e:xopbb [2025/02/12 23:47] (current) – jfpothier
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-====== XopBB ======+====== The Type III Effector XopBB from //Xanthomonas// ======
  
-Author: John Doe\\ +Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]
-Reviewer: Jane Doe+
  
 Class: XopBB\\ Class: XopBB\\
 Family: XopBB\\ Family: XopBB\\
-Prototype: ELAGFFLI_02299 (//Xanthomonas ////hortorum// pv. //pelargonii//; strain 305) +Prototype: ELAGFFLI_02299 (//Xanthomonas hortorum// pv. //pelargonii//; strain 305)\\ 
- +GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/CAD0333498.1|CAD0333498]] (332 aa)\\ 
-GanBank ID: CAD0333498 (332 aa)\\ +RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_251761698.1|WP_251761698.1]] (220 aa, corresponding to the C-terminal domain)\\ 
-3D structure:+3D structure: Unknown
  
 ===== Biological function ===== ===== Biological function =====
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria //T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBB ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).+To examine the translocation of the predicted T3E, a reporter system based on the delivery of a truncated form of the //Xanthomonas euvesicatoria// T3E AvrBs2 (amino acids 62–574) into susceptible plant cells was utilized. AvrBs2<sub>62–574</sub> lacks the type 3 translocation signal, but is sufficient to elicit a hypersensitive response (HR) in plants expressing the //Bs2// resistance gene. A fusion of the XopBB ORF, including 24 bp upstream of its ATG start codon, with AvrBs2<sub>62–574</sub> elicited HR response in the pepper line ECW20R, which encodes a functional //Bs2// resistance gene (Wagner //et al.//, 2023).
 === Regulation === === Regulation ===
  
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 === In xanthomonads === === In xanthomonads ===
  
-Yes, //X. campestris// and //X. hydrangeae// (Wagner //et al.//, 2023).+Yes, //X. arboricola// and //X. hydrangeae// (Wagner //et al.//, 2023).
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
-Yes, //Acidovorax citrulli// (Wagner //et al.//, 2023).+Yes, in species of //Acidovorax// (//e.g.//, //A. avenae//, //A. citrulli//, //A. oryzae//) (Wagner //et al.//, 2023). XopBB is distantly related to HopF2 (syn. AvrPphF) from the //Pseudomonas syringae// species complex, e.g. //P. amygdali//, //P. cannabina// (ca. 30 % amino acid sequence identity).
  
 ===== References ===== ===== References =====
bacteria/t3e/xopbb.1702910277.txt.gz · Last modified: 2023/12/18 14:37 by rkoebnik

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