EuroXanth DokuWiki

User Tools

Site Tools

Trace:
bacteria:t3e:xopba

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
Next revision		Previous revision
bacteria:t3e:xopba [2025/11/15 14:03] – jfpothierbacteria:t3e:xopba [2025/11/24 14:31] (current) – jfpothier
Line 2: Line 2:
  
 Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\ Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\
-Internal reviewer: Anna Passelergue+Internal reviewer: Anna Passelergue\\
  
 Class: XopBA\\ Class: XopBA\\
Line 17: Line 17:
  
 The homolog in //Xanthomonas graminis// pv. //arrhenatheri// strain LMG 727 with 77% sequence identity (KM317_RS05475), XopBA<sub>//Xga//LMG727</sub>, was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025) The homolog in //Xanthomonas graminis// pv. //arrhenatheri// strain LMG 727 with 77% sequence identity (KM317_RS05475), XopBA<sub>//Xga//LMG727</sub>, was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025)
-=== (Experimental) evidence for being a T3E === 
  
 +=== (Experimental) evidence for being a T3E ===
 XopBA<sub>//Xga//LMG727</sub> was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013; Passelergue, 2025). XopBA<sub>//Xga//LMG727</sub> was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013; Passelergue, 2025).
 +
 === Regulation === === Regulation ===
 +The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub>‐YANNNT) suggests that the //xopBA<sub>Xga LMG727</sub>// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006).
  
-The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopBA<sub>Xga</sub>// <sub>LMG727</sub>// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006). 
 === Phenotypes === === Phenotypes ===
- 
 Ectopic expression of the distant homolog in //E. coli//, EspW, results in formation of unique membrane protrusions (Sandu //et al.//, 2017) Ectopic expression of the distant homolog in //E. coli//, EspW, results in formation of unique membrane protrusions (Sandu //et al.//, 2017)
-=== Localization === 
  
 +=== Localization ===
 The distant homolog in //E. coli//, EspW, and Kif15 colocalized in cotransfected cells, while ectopically expressed Kif15 localized to the actin pedestals following EHEC infection (Sandu //et al.//, 2017). The distant homolog in //E. coli//, EspW, and Kif15 colocalized in cotransfected cells, while ectopically expressed Kif15 localized to the actin pedestals following EHEC infection (Sandu //et al.//, 2017).
-=== Enzymatic function === 
  
 +=== Enzymatic function ===
 The distant homolog in //E. coli//, EspW, modulates actin dynamics in a Rac1-dependent manner (Sandu //et al.//, 2017). The distant homolog in //E. coli//, EspW, modulates actin dynamics in a Rac1-dependent manner (Sandu //et al.//, 2017).
-=== Interaction partners === 
  
 +=== Interaction partners ===
 The distant homolog in //E. coli//, EspW, was found to interact with the motor protein Kif15 in a yeast two-hybrid screen (Sandu //et al.//, 2017). The distant homolog in //E. coli//, EspW, was found to interact with the motor protein Kif15 in a yeast two-hybrid screen (Sandu //et al.//, 2017).
  
Line 40: Line 40:
 === In xanthomonads === === In xanthomonads ===
  
-Yes (//e.g.//, //X. euvesicatoria, X//. //axonopodis//, //X. campestris//, //X. vasicola//, //X. citri, X//.// phaseoli//,// ////X. arboricola//, //X. graminis//, //X. transclucens, X. hortorum//, //X. populi//,// X. bromi//,// X//. //nasturtii//,// X//. //hyacinthi//)+Yes (//e.g.//, //X. euvesicatoria, X//. //axonopodis//, //X. campestris//, //X. vasicola//, //X. citri, //X. phaseoli//,//X. arboricola//, //X. graminis//, //X. transclucens//, //X. hortorum//, //X. populi//, //X. bromi//, //X. nasturtii//, //X 
 +. hyacinthi//)
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
-Yes (//e.g.//, //Pseudomonas// spp.,// Erwinia// spp., //Robbsia andropogonis//, //Acidovorax/////Paracidovorax //spp.)+Yes (//e.g.//, //Pseudomonas// spp., //Erwinia// spp., //Robbsia andropogonis//, //Acidovorax/////Paracidovorax// spp.)
  
 ===== References ===== ===== References =====
bacteria/t3e/xopba.1763215418.txt.gz · Last modified: 2025/11/15 14:03 by jfpothier

Page Tools

Except where otherwise noted, content on this wiki is licensed under the following license: CC Attribution-Share Alike 4.0 International
CC Attribution-Share Alike 4.0 International Donate Powered by PHP Valid HTML5 Valid CSS Driven by DokuWiki