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bacteria:t3e:xopba [2025/08/04 10:19] – [The Type III Effector XopBA from //Xanthomonas//] rkoebnikbacteria:t3e:xopba [2025/11/24 14:31] (current) – jfpothier
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 Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\ Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\
-Internal reviewer: Anna Passelergue+Internal reviewer: Anna Passelergue\\
  
 Class: XopBA\\ Class: XopBA\\
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 XopBA<sub>//Xee//66b</sub> was discovered by homology to HopW/HopPmaA in //Pseudomonas syringae// (70% aa sequence identity) and EspW in //Escherichia coli// (30% aa sequence identity) (Stavrinides & Guttman, 2004; Sandu et al., 2017). XopBA<sub>//Xee//66b</sub> was discovered by homology to HopW/HopPmaA in //Pseudomonas syringae// (70% aa sequence identity) and EspW in //Escherichia coli// (30% aa sequence identity) (Stavrinides & Guttman, 2004; Sandu et al., 2017).
  
-XopBA<sub>//Xga//LMG727</sub> was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025)+The homolog in //Xanthomonas graminis// pv. //arrhenatheri// strain LMG 727 with 77% sequence identity (KM317_RS05475), XopBA<sub>//Xga//LMG727</sub>, was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025) 
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
 +XopBA<sub>//Xga//LMG727</sub> was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013; Passelergue, 2025).
  
-XopBA<sub>//Xee//66b</sub>: No evidence. 
- 
-XopBA<sub>//Xga//LMG727</sub> was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013). 
 === Regulation === === Regulation ===
 +The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub>‐YANNNT) suggests that the //xopBA<sub>Xga LMG727</sub>// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006).
  
-XopBA<sub>//Xee//66b</sub>: Unknown. 
- 
-XopBA<sub>//Xga//LMG727</sub>: The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopBA<sub>Xga</sub> // <sub>LMG727</sub> //<sup> </sup>  // gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006). 
 === Phenotypes === === Phenotypes ===
- 
 Ectopic expression of the distant homolog in //E. coli//, EspW, results in formation of unique membrane protrusions (Sandu //et al.//, 2017) Ectopic expression of the distant homolog in //E. coli//, EspW, results in formation of unique membrane protrusions (Sandu //et al.//, 2017)
 +
 === Localization === === Localization ===
 +The distant homolog in //E. coli//, EspW, and Kif15 colocalized in cotransfected cells, while ectopically expressed Kif15 localized to the actin pedestals following EHEC infection (Sandu //et al.//, 2017).
  
-The distant homolog in //E. coli//, EspW, and Kif15 colocalized in cotransfected cells, while ectopically expressed Kif15 localized to the actin pedestals following EHEC infection (Sandu //et al.//, 2017). 
 === Enzymatic function === === Enzymatic function ===
 +The distant homolog in //E. coli//, EspW, modulates actin dynamics in a Rac1-dependent manner (Sandu //et al.//, 2017).
  
-The distant homolog in //E. coli//, EspW, modulates actin dynamics in a Rac1-dependent manner (Sandu //et al.//, 2017). 
 === Interaction partners === === Interaction partners ===
- 
 The distant homolog in //E. coli//, EspW, was found to interact with the motor protein Kif15 in a yeast two-hybrid screen (Sandu //et al.//, 2017). The distant homolog in //E. coli//, EspW, was found to interact with the motor protein Kif15 in a yeast two-hybrid screen (Sandu //et al.//, 2017).
  
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 === In xanthomonads === === In xanthomonads ===
  
-XopBA<sub>//Xee//66b</sub>: Yes (//e.g.//, //X. euvesicatoria, X. citri, X. transclucens, X. vasicola//). +Yes (//e.g.//, //X. euvesicatoria, X//. //axonopodis//, //X. campestris//, //X. vasicola//, //X. citri, //X. phaseoli//,//X. arboricola//, //X. graminis//, //X. transclucens//, //X. hortorum//, //X. populi//, //X. bromi//, //X. nasturtii//, //X 
- +. hyacinthi//)
-XopBA<sub>//Xga//LMG727</sub>: Yes (//e.g.//, //X. translucens//, //X. hortorum//, //X. vasicola//, //X. populi//,// X. euvesicatoria//, //X. arboricola//, //X//. //axonopodis//, //X. campestris//, //X. bromi//,// X//. //nasturtii//,// X//.// phaseoli//,// X//.// cissicola//, //X. perforans//,// X//. //hyacinthi//).+
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
-XopBA<sub>//Xee//66b</sub>: Yes (//Acidovorax// spp., //Erwinia// spp., //Pseudomonas syringae//). +Yes (//e.g.//, //Pseudomonas// spp., //Erwinia// spp., //Robbsia andropogonis//, //Acidovorax/////Paracidovorax// spp.)
- +
-XopBA<sub>//Xga//LMG727</sub>: Yes (//e.g.//, //Pseudomonas //spp.,// Erwinia //spp., //Robbsia andropogonis//)+
  
 ===== References ===== ===== References =====
bacteria/t3e/xopba.1754299179.txt.gz · Last modified: 2025/08/04 10:19 by rkoebnik

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