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bacteria:t3e:xopba

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bacteria:t3e:xopba [2025/07/31 12:57] – [Biological function] apasselerguebacteria:t3e:xopba [2025/11/24 14:31] (current) – jfpothier
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 ====== The Type III Effector XopBA from //Xanthomonas// ====== ====== The Type III Effector XopBA from //Xanthomonas// ======
  
-Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]], Anna Passelergue +Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\ 
- +Internal reviewer: Anna Passelergue\\
-Internal reviewer: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]+
  
 Class: XopBA\\ Class: XopBA\\
 Family: XopBA\\ Family: XopBA\\
-Prototype: XopBA (XopBA <font inherit/inherit;;#95a5a6;;inherit><sup>1</sup></font>  ) (//Xanthomonas euvesicatoria// pv. //euvesicatoria//; strain 66b), KM317_RS05475 (XopBA <font inherit/inherit;;#95a5a6;;inherit><sup>2</sup></font>  ) (Xanthomonas graminis pv. arrhenatheri; strain LMG 727)\\ +Prototype: XopBA<sub>//Xee//66b</sub> (//Xanthomonas euvesicatoria// pv. //euvesicatoria//; strain 66b)\\ 
-RefSeq ID: (XopBA <font inherit/inherit;;#95a5a6;;inherit><sup>1</sup></font>  ) [[https://www.ncbi.nlm.nih.gov/protein/WP_227478587.1|WP_227478587.1]] (500 aa),(XopBA <font inherit/inherit;;#95a5a6;;inherit><sup>2</sup></font>  ) [[https://www.ncbi.nlm.nih.gov/protein/WP_231108156.1|WP_231108156.1]] (499 aa)\\+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_227478587.1|WP_227478587.1]] (500 aa)\\
 3D structure: Unknown 3D structure: Unknown
  
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 === How discovered? === === How discovered? ===
  
-XopBA +XopBA<sub>//Xee//66b</sub> was discovered by homology to HopW/HopPmaA in //Pseudomonas syringae// (70% aa sequence identity) and EspW in //Escherichia coli// (30% aa sequence identity) (Stavrinides & Guttman, 2004; Sandu et al., 2017). 
- <font inherit/inherit;;#95a5a6;;inherit><sup>1</sup></font>  was discovered by homology to HopW/HopPmaA in //Pseudomonas syringae// (70% aa sequence identity) and EspW in //Escherichia coli// (30% aa sequence identity) (Stavrinides & Guttman, 2004; Sandu et al., 2017).+ 
 +The homolog in //Xanthomonas graminis// pv. //arrhenatheri// strain LMG 727 with 77% sequence identity (KM317_RS05475), XopBA<sub>//Xga//LMG727</sub>, was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025)
  
-XopBA 
- <font inherit/inherit;;#95a5a6;;inherit><sup>2</sup></font>  was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025) 
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
 +XopBA<sub>//Xga//LMG727</sub> was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013; Passelergue, 2025).
  
-XopBA 
- <font inherit/inherit;;#95a5a6;;inherit><sup>1</sup></font>  : No evidence. 
- 
-XopBA 
- <font inherit/inherit;;#95a5a6;;inherit><sup>2 </sup></font>  was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013). 
 === Regulation === === Regulation ===
 +The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub>‐YANNNT) suggests that the //xopBA<sub>Xga LMG727</sub>// gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006).
  
-XopBA 
- <font inherit/inherit;;#95a5a6;;inherit><sup>1</sup></font>  : Unknown. 
- 
-The presence of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) suggests that the //xopBA<sup> 
- <font inherit/inherit;;#95a5a6;;inherit>2</font>  </sup>   //  gene is under control of HrpG and HrpX (Wengelnik & Bonas, 1996; Wengelnik //et al.//, 1996; Koebnik //et al.//, 2006). 
 === Phenotypes === === Phenotypes ===
 +Ectopic expression of the distant homolog in //E. coli//, EspW, results in formation of unique membrane protrusions (Sandu //et al.//, 2017)
  
-XopBA  <sup>1</sup>  : Ectopic expression of the distant homolog in //E. coli//, EspW, results in formation of unique membrane protrusions (Sandu //et al.//, 2017) 
 === Localization === === Localization ===
 +The distant homolog in //E. coli//, EspW, and Kif15 colocalized in cotransfected cells, while ectopically expressed Kif15 localized to the actin pedestals following EHEC infection (Sandu //et al.//, 2017).
  
-XopBA  <sup>1</sup>  : The distant homolog in //E. coli//, EspW, and Kif15 colocalized in cotransfected cells, while ectopically expressed Kif15 localized to the actin pedestals following EHEC infection (Sandu //et al.//, 2017). 
 === Enzymatic function === === Enzymatic function ===
 +The distant homolog in //E. coli//, EspW, modulates actin dynamics in a Rac1-dependent manner (Sandu //et al.//, 2017).
  
-XopBA  <sup>1</sup>  : The distant homolog in //E. coli//, EspW, modulates actin dynamics in a Rac1-dependent manner (Sandu //et al.//, 2017). 
 === Interaction partners === === Interaction partners ===
- +The distant homolog in //E. coli//, EspW, was found to interact with the motor protein Kif15 in a yeast two-hybrid screen (Sandu //et al.//, 2017).
-XopBA <font inherit/inherit;;#95a5a6;;inherit><sup>1</sup></font> : The distant homolog in //E. coli//, EspW, was found to interact with the motor protein Kif15 in a yeast two-hybrid screen (Sandu //et al.//, 2017).+
  
 ===== Conservation ===== ===== Conservation =====
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 === In xanthomonads === === In xanthomonads ===
  
-XopBA <font inherit/inherit;;#95a5a6;;inherit><sup>1</sup></font>  : Yes (//e.g.//, //X. euvesicatoria, X. citri, X. transclucens, X. vasicola//). +Yes (//e.g.//, //X. euvesicatoria, X//. //axonopodis//, //X. campestris//, //X. vasicola//, //X. citri, //X. phaseoli//,//X. arboricola//, //X. graminis//, //X. transclucens//, //X. hortorum//, //X. populi//, //X. bromi//, //X. nasturtii//, //X 
- +. hyacinthi//)
-XopBA <font inherit/inherit;;#95a5a6;;inherit><sup>2</sup></font>  : Yes (//e.g.//, //X. translucens//, //X. hortorum//, //X. vasicola//, //X. populi//,// X. euvesicatoria//, //X. arboricola//, //X//. //axonopodis//, //X. campestris//, //X. bromi//,// X//. //nasturtii//,// X//.// phaseoli//,// X//.// cissicola//, //X. perforans//,// X//. //hyacinthi//).+
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
-XopBA <font inherit/inherit;;#95a5a6;;inherit><sup>1</sup></font>  : Yes (//Acidovorax// spp., //Erwinia// spp., //Pseudomonas syringae//).+Yes (//e.g.//, //Pseudomonas// spp., //Erwinia// spp., //Robbsia andropogonis//, //Acidovorax/////Paracidovorax// spp.)
  
-XopBA <font inherit/inherit;;#95a5a6;;inherit><sup>2</sup></font>  : Yes (//e.g.//, //Pseudomonas //spp.,// Erwinia //spp., //Robbsia andropogonis//) 
 ===== References ===== ===== References =====
  
bacteria/t3e/xopba.1753963072.txt.gz · Last modified: 2025/07/31 12:57 by apasselergue

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