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--- bacteria:t3e:xopaz [2023/05/31 14:54] – [Biological function] rkoebnik
+++ bacteria:t3e:xopaz [2025/01/27 22:27] (current) – [Conservation] jfpothier
@@ Line 1: / Line 1: @@
-====== XopAZ ======
+====== The Type III Effector XopAZ from //Xanthomonas// ======
-Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\
+Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]
-Internal reviewer:\\
-Expert reviewer
 Class: XopAZ\\
@@ Line 16: / Line 14: @@
 === How discovered? ===
-A cosmid library was created from genomic DNA of //X. cynarae// and the cosmid clone library was screened in //X. perforans// strain ME24. The transconjugants were infiltrated into tomato to identify the clones which resulted in rapid necrosis, characteristic of an HR. Three of the clones elicited HRs in tomato. One of the clones was subcloned and the fragments were tested for ability to elicit an HR in tomato. One of the subclones elicited an HR and was determined to contain a 510 bp open reading frame (ORF). BLAST analysis of the nucleotide sequence revealed 98.6% sequence identity with a peptidylprolyl isomerase gene in //X. gardneri// strain ICMP 7893 (Kara //et al.//, 20017). This effector protein, which was called XopAZ in a poster presented at APS Meeting in 2017, had 169 aa with 12 extra amino acids at the N terminus (MHSTRSPSWRFP) (Kara //et al.//, 20017).
+A cosmid library was created from genomic DNA of //X. cynarae// and the cosmid clone library was screened in //X. perforans// strain ME24. The transconjugants were infiltrated into tomato to identify the clones which resulted in rapid necrosis, characteristic of an HR. Three of the clones elicited HRs in tomato. One of the clones was subcloned and the fragments were tested for ability to elicit an HR in tomato. One of the subclones elicited an HR and was determined to contain a 510 bp open reading frame (ORF). BLAST analysis of the nucleotide sequence revealed 98.6% sequence identity with a peptidylprolyl isomerase gene in //X. gardneri// strain ICMP 7893 (Kara //et al.//, 2017). This effector protein, which was called XopAZ in a poster presented at APS Meeting in 2017, had 169 aa with 12 extra amino acids at the N terminus (MHSTRSPSWRFP) (Kara //et al.//, 2017).
 === (Experimental) evidence for being a T3E ===
-Whether the protein is an effector delivered by the type III secretion system was tested using a reporter system in which 70 amino acids at the N-terminus of the candidate gene was fused to the C-terminus of AvrBs2 as described previously (Roden et al., 2004). When a bacterial suspension of the //X. euvesicatoria// strain Ted 3 that did not contain //avrBs2//, but that was carrying the vector encoding the fusion protein, was infiltrated into pepper leaves containing //Bs2// (ECW-20R) and pepper leaves without //Bs2// (ECW), an HR was observed only in ECW-20R.
+Whether the protein is an effector delivered by the type III secretion system was tested using a reporter system in which 70 amino acids at the N-terminus of the candidate gene was fused to the C-terminus of AvrBs2 as described previously (Roden //et al.//, 2004). When a bacterial suspension of the //X. euvesicatoria// strain Ted 3 that did not contain //avrBs2//, but that was carrying the vector encoding the fusion protein, was infiltrated into pepper leaves containing //Bs2// (ECW-20R) and pepper leaves without //Bs2// (ECW), an HR was observed only in ECW-20R.
 === Regulation ===
@@ Line 36: / Line 34: @@
 === In xanthomonads ===
-Universally conserved protein.
+Universally conserved protein, //e.g.//, //Xanthomonas melonis// (Ramnarine //et al.//, 2022).
 === In other plant pathogens/symbionts ===

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