bacteria:t3e:xopaz
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| bacteria:t3e:xopaz [2020/07/09 10:46] – rkoebnik | bacteria:t3e:xopaz [2025/01/27 22:27] (current) – [Conservation] jfpothier | ||
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| - | ====== XopAZ ====== | + | ====== |
| - | Author: | + | Author: |
| - | Reviewer: Jane Doe | + | |
| Class: XopAZ\\ | Class: XopAZ\\ | ||
| Family: XopAZ\\ | Family: XopAZ\\ | ||
| - | Prototype: | + | Prototype: |
| - | RefSeq ID:\\ | + | GenBank ID: [[https:// |
| + | RefSeq ID: [[https:// | ||
| 3D structure: Unknown | 3D structure: Unknown | ||
| Line 14: | Line 14: | ||
| === How discovered? === | === How discovered? === | ||
| + | A cosmid library was created from genomic DNA of //X. cynarae// and the cosmid clone library was screened in //X. perforans// strain ME24. The transconjugants were infiltrated into tomato to identify the clones which resulted in rapid necrosis, characteristic of an HR. Three of the clones elicited HRs in tomato. One of the clones was subcloned and the fragments were tested for ability to elicit an HR in tomato. One of the subclones elicited an HR and was determined to contain a 510 bp open reading frame (ORF). BLAST analysis of the nucleotide sequence revealed 98.6% sequence identity with a peptidylprolyl isomerase gene in //X. gardneri// strain ICMP 7893 (Kara //et al.//, 2017). This effector protein, which was called XopAZ in a poster presented at APS Meeting in 2017, had 169 aa with 12 extra amino acids at the N terminus (MHSTRSPSWRFP) (Kara //et al.//, 2017). | ||
| === (Experimental) evidence for being a T3E === | === (Experimental) evidence for being a T3E === | ||
| + | Whether the protein is an effector delivered by the type III secretion system was tested using a reporter system in which 70 amino acids at the N-terminus of the candidate gene was fused to the C-terminus of AvrBs2 as described previously (Roden //et al.//, 2004). When a bacterial suspension of the //X. euvesicatoria// | ||
| === Regulation === | === Regulation === | ||
| Line 23: | Line 25: | ||
| === Enzymatic function === | === Enzymatic function === | ||
| + | |||
| + | Predicted peptidylprolyl isomerase. | ||
| === Interaction partners === | === Interaction partners === | ||
| Line 30: | Line 34: | ||
| === In xanthomonads === | === In xanthomonads === | ||
| + | Universally conserved protein, //e.g.//, // | ||
| === In other plant pathogens/ | === In other plant pathogens/ | ||
| + | |||
| + | Universally conserved protein. | ||
| ===== References ===== | ===== References ===== | ||
| + | |||
| + | Kara S (2016). Characterization of a new type three secretion effector in // | ||
| + | |||
| + | Kara S, Timilsina S, Jacques MA, Potnis N, Vallad G, Fischer-Le Saux M, Hurlbert JC, Minsavage GV, Jones JB (2017). A novel type III Xop effector in // | ||
| + | |||
| + | Ramnarine SDBJ, Jayaraman J, Ramsubhag A (2022). Comparative genomics of the black rot pathogen // | ||
| + | |||
| + | Roden JA, Belt B, Ross JB, Tachibana T, Vargas J, Mudgett MB (2004). A genetic screen to isolate type III effectors translocated into pepper cells during // | ||
bacteria/t3e/xopaz.1594288003.txt.gz · Last modified: 2023/01/09 10:20 (external edit)
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