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--- bacteria:t3e:xopao [2023/01/09 10:20] – external edit 127.0.0.1
+++ bacteria:t3e:xopao [2024/12/16 14:45] (current) – [The Type III Effector XopAO from //Xanthomonas//] rkoebnik
@@ Line 1: / Line 1: @@
-====== XopAO ======
+====== The Type III Effector XopAO from //Xanthomonas// ======
-Author: John Doe\\
+Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]
-Reviewer: Jane Doe
 Class: XopAO\\
 Family: XopAO\\
-Prototype: Xop? (//Xanthomonas//; strain)\\
+Prototype: XGA_1250 (//Xanthomonas hortorum// pv. //gardneri//, ex. //Xanthomonas gardneri//; strain 101 = ATCC 19865)\\
-RefSeq ID:\\
+GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/EGD20072.1|EGD20072.1]] (206 aa)\\
+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_162267513.1|WP_162267513.1]] (207 aa)\\
 D structure: Unknown
@@ Line 14: / Line 14: @@
 === How discovered? ===
+XopAO was predicted to be a type 3 effector based on homology to AvrRpm1, a type 3 effector from //Pseudomonas syringae// (Potnis //et al.//, 2011).
 === (Experimental) evidence for being a T3E ===
+T3SS-mediated translocation of XopAO was assessed by constructing fusion genes with the C-terminal end of AvrBs2 coding sequence (//avrBs2// <sub>62-574</sub>) in a race 6 strain of //X. euvesicatoria//. Translocation was measured in pepper cv. ECW 20R, containing the resistance gene //Bs2//. //xopAO// was demonstrated to direct AvrBs2-specific hypersensitive reactions in ECW 20R (Potnis //et al.//, 2011).
 === Regulation ===

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