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bacteria:t3e:xopan

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bacteria:t3e:xopan [2023/10/04 17:22] – [References] rkoebnikbacteria:t3e:xopan [2025/07/04 23:19] (current) – jfpothier
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-====== XopAN (replaced by XopAV) ======+====== The Type III Effector XopAN from //Xanthomonas// (replaced by XopAV) ======
  
 Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\ Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\
-Internal reviewer:\\ 
-Expert reviewer: 
  
 Class: XopAN\\ Class: XopAN\\
 Family: XopAN\\ Family: XopAN\\
-Prototype: XopAN (//Xanthomonas//; strain)\\ +Prototype: XopAN (//Xanthomonas campestris// pv. //campestris//)\\
-GenBank ID:\\ +
-RefSeq ID:\\+
 3D structure: Unknown 3D structure: Unknown
  
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 === How discovered? === === How discovered? ===
  
-XopAN was originally reserved for a type 3 effector from //X. campestris// pv. //campestris//, which turned out to be related to [[https://www.ncbi.nlm.nih.gov/protein/AAY50220.1|XC_3176]], a protein that was identified as a type 3 effector and nominated XopAY (see below; Yang et al., 2015). The N-terminal portion of XC_3176 is similar to XCV1197 and the C-terminal portion is similar to XCV1198. XCV1197 was shown to contain a type 3 secretion signal and was called XopAV, now classified as XopAV1 (Teper et al., 2016 ), whereas XC_3176 was classified as XopAV2.+XopAN was originally reserved for a type 3 effector from //X. campestris// pv. //campestris//, which turned out to be related to [[https://www.ncbi.nlm.nih.gov/protein/AAY50220.1|XC_3176]], a protein that was identified as a type 3 effector and nominated XopAY (see below; Yang et al., 2015). The N-terminal portion of [[https://www.ncbi.nlm.nih.gov/protein/AAY50220.1|XC_3176]] is similar to [[https://www.ncbi.nlm.nih.gov/protein/CAJ22828.1|XCV1197]] and the C-terminal portion is similar to [[https://www.ncbi.nlm.nih.gov/protein/CAJ22829.1|XCV1198]]. [[https://www.ncbi.nlm.nih.gov/protein/CAJ22828.1|XCV1197]] was shown to contain a type 3 secretion signal and was called [[:bacteria:t3e:xopav|XopAV]], now classified as XopAV1 (Teper et al., 2016 ), whereas XC_3176 was classified as XopAV2. For more information, see also [[:bacteria:t3e:xopay|XopAY]].
  
 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-The promoter and signal region of XC_3176 were fused to the HR-inducing AvrBs1 C-terminal domain lack of 58 N-terminal amino acid residues. This construct elicited an hypersensitive response on the pepper ECW-10R containing the corresponding resistance gene// Bs1// in a //hrcV//-dependent manner (Yang //et al.//, 2015).+The promoter and signal region of XC_3176 were fused to the HR-inducing AvrBs1 C-terminal domain lack of 58 N-terminal amino acid residues. This construct elicited an hypersensitive response on the pepper ECW-10R containing the corresponding resistance gene //Bs1// in a //hrcV//-dependent manner (Yang //et al.//, 2015). 
 === Regulation === === Regulation ===
  
 A reporter GUS fusion of XC_3176 displayed GUS activities, which were significantly lower in the mutant strains Δ//hrpX// and Δ//hrpG// than that in the wild type //X. campestris// pv. //campestris// strain (Yang //et al.//, 2015). A reporter GUS fusion of XC_3176 displayed GUS activities, which were significantly lower in the mutant strains Δ//hrpX// and Δ//hrpG// than that in the wild type //X. campestris// pv. //campestris// strain (Yang //et al.//, 2015).
 +
 === Phenotypes === === Phenotypes ===
  
 XC_3176 was found to be required for the full virulence of //X. campestris// pv. //campestris// strain 8004 when assayed on Chinese radish by the leaf-clipping method (Yang //et al.//, 2015). XC_3176 was found to be required for the full virulence of //X. campestris// pv. //campestris// strain 8004 when assayed on Chinese radish by the leaf-clipping method (Yang //et al.//, 2015).
 +
 === Localization === === Localization ===
  
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 Yes (e.g. //X. campestris//, //X. hortorum//, //X. arboricola//, //X. fragariae//) Yes (e.g. //X. campestris//, //X. hortorum//, //X. arboricola//, //X. fragariae//)
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
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 Yang L, Su H, Yang F, Jian H, Zhou M, Jiang W, Jiang B (2015). Identification of a new type III effector XC3176 in //Xanthomonas campestris// pv. //campestris//. Wei Sheng Wu Xue Bao 55: 1264-1272. PubMed ID: [[https://pubmed.ncbi.nlm.nih.gov/26939454/|26939454]]. Article in Chinese. Yang L, Su H, Yang F, Jian H, Zhou M, Jiang W, Jiang B (2015). Identification of a new type III effector XC3176 in //Xanthomonas campestris// pv. //campestris//. Wei Sheng Wu Xue Bao 55: 1264-1272. PubMed ID: [[https://pubmed.ncbi.nlm.nih.gov/26939454/|26939454]]. Article in Chinese.
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xopan.1696436542.txt.gz · Last modified: 2023/10/04 17:22 by rkoebnik

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