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--- bacteria:t3e:xopan [2023/10/03 07:55] – [XopAN] rkoebnik
+++ bacteria:t3e:xopan [2025/02/12 23:35] (current) – jfpothier
@@ Line 1: / Line 1: @@
-====== XopAN ======
+====== The Type III Effector XopAN from //Xanthomonas// (replaced by XopAV) ======
-Author: John Doe\\
+Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]
-Internal reviewer: Jane Doe \\
-Expert reviewer: **WANTED!**
 Class: XopAN\\
 Family: XopAN\\
-Prototype: XopAN (//Xanthomonas//; strain)\\
+Prototype: XopAN (//Xanthomonas campestris// pv. //campestris//)\\
-GenBank ID:\\
-RefSeq ID:\\
 D structure: Unknown
@@ Line 15: / Line 11: @@
 === How discovered? ===
+XopAN was originally reserved for a type 3 effector from //X. campestris// pv. //campestris//, which turned out to be related to [[https://www.ncbi.nlm.nih.gov/protein/AAY50220.1|XC_3176]], a protein that was identified as a type 3 effector and nominated XopAY (see below; Yang et al., 2015). The N-terminal portion of [[https://www.ncbi.nlm.nih.gov/protein/AAY50220.1|XC_3176]] is similar to [[https://www.ncbi.nlm.nih.gov/protein/CAJ22828.1|XCV1197]] and the C-terminal portion is similar to [[https://www.ncbi.nlm.nih.gov/protein/CAJ22829.1|XCV1198]]. [[https://www.ncbi.nlm.nih.gov/protein/CAJ22828.1|XCV1197]] was shown to contain a type 3 secretion signal and was called [[:bacteria:t3e:xopav|XopAV]], now classified as XopAV1 (Teper et al., 2016 ), whereas XC_3176 was classified as XopAV2. For more information, see also [[:bacteria:t3e:xopay|XopAY]].
 === (Experimental) evidence for being a T3E ===
+The promoter and signal region of XC_3176 were fused to the HR-inducing AvrBs1 C-terminal domain lack of 58 N-terminal amino acid residues. This construct elicited an hypersensitive response on the pepper ECW-10R containing the corresponding resistance gene //Bs1// in a //hrcV//-dependent manner (Yang //et al.//, 2015).
 === Regulation ===
+A reporter GUS fusion of XC_3176 displayed GUS activities, which were significantly lower in the mutant strains Δ//hrpX// and Δ//hrpG// than that in the wild type //X. campestris// pv. //campestris// strain (Yang //et al.//, 2015).
 === Phenotypes ===
+XC_3176 was found to be required for the full virulence of //X. campestris// pv. //campestris// strain 8004 when assayed on Chinese radish by the leaf-clipping method (Yang //et al.//, 2015).
 === Localization ===
+Unknown
 === Enzymatic function ===
+Unknown
 === Interaction partners ===
+Unknown
 ===== Conservation =====
@@ Line 32: / Line 39: @@
 === In xanthomonads ===
+Yes (e.g. //X. campestris//, //X. hortorum//, //X. arboricola//, //X. fragariae//)
 === In other plant pathogens/symbionts ===
+No
 ===== References =====
+Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel //Xanthomonas euvesicatoria// type III effector proteins by a machine-learning approach. Mol. Plant Pathol. 17: 398-411. DOI: [[https://doi.org/10.1111/mpp.12288|10.1111/mpp.12288]]
+Yang L, Su H, Yang F, Jian H, Zhou M, Jiang W, Jiang B (2015). Identification of a new type III effector XC3176 in //Xanthomonas campestris// pv. //campestris//. Wei Sheng Wu Xue Bao 55: 1264-1272. PubMed ID: [[https://pubmed.ncbi.nlm.nih.gov/26939454/|26939454]]. Article in Chinese.

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