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bacteria:t3e:xopam

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bacteria:t3e:xopam [2026/10/29 15:08] – [References] rkoebnikbacteria:t3e:xopam [2026/10/29 15:09] (current) – [Biological function] rkoebnik
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 The //xopAM// gene in //X. campstris// pv. //campestris// strain 8004 (Xcc8004) was identified as a candidate T3E due to the presence of a PIP box (TTCGB-N<sub>15</sub>-TTCGB) within the upstream sequence of its putative translational start codon (Jiang //et al.//, 2009). The //xopAM// gene in //X. campstris// pv. //campestris// strain 8004 (Xcc8004) was identified as a candidate T3E due to the presence of a PIP box (TTCGB-N<sub>15</sub>-TTCGB) within the upstream sequence of its putative translational start codon (Jiang //et al.//, 2009).
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
 Using the HR-inducing domain AvrBs1<sub>59-445</sub> as a type-3 translocation reporter, the N-terminal region of XopAM<sub>Xcc8004</sub> was found to act as a type 3 secretion signal, since the translational reporter fusion triggered a //Bs1//-specific hypersensitive response in a //hrpF//- and //hpaB//-dependent manner (Jiang //et al.//, 2009). Using the HR-inducing domain AvrBs1<sub>59-445</sub> as a type-3 translocation reporter, the N-terminal region of XopAM<sub>Xcc8004</sub> was found to act as a type 3 secretion signal, since the translational reporter fusion triggered a //Bs1//-specific hypersensitive response in a //hrpF//- and //hpaB//-dependent manner (Jiang //et al.//, 2009).
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 === Regulation === === Regulation ===
  
 Using a promoterless beta-glucuronidase (//gus//) gene as a transcriptional reporter, //xopAM// <sub>Xcc8004</sub> was found to be under control of HrpG and HrpX (Jiang //et al.//, 2009). Using a promoterless beta-glucuronidase (//gus//) gene as a transcriptional reporter, //xopAM// <sub>Xcc8004</sub> was found to be under control of HrpG and HrpX (Jiang //et al.//, 2009).
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 === Phenotypes === === Phenotypes ===
  
-Mutagenesis of type III effectors in //X. campestris// pv. //campestris// (//Xcc//) confirmed that //xopAM// functions as a second avirulence gene on plants of the Arabidopsis Col-0 ecotype (Guy //et al.//, 2013).+Mutagenesis of type III effectors in //X. campestris// pv. //campestris// (//Xcc//) confirmed that //xopAM// functions as a second avirulence gene on plants of the //Arabidopsis// Col-0 ecotype (Guy //et al.//, 2013).
  
 Deletion of //xopAM// from //Xcc// reduced its virulence in cruciferous crops but increased virulence in Arabidopsis (//Arabidopsis thaliana//) Col-0, indicating that XopAM may perform opposite functions depending on the host species (Xie //et al.//, 2023). Deletion of //xopAM// from //Xcc// reduced its virulence in cruciferous crops but increased virulence in Arabidopsis (//Arabidopsis thaliana//) Col-0, indicating that XopAM may perform opposite functions depending on the host species (Xie //et al.//, 2023).
  
 +Deletion of //xopAM<sub>Xcc</sub> // increased disease indices by more than 10% in cabbage line G1180, while bacterial counts also increased (Chen //et al.//, 2026).
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 +Reverse transcription quantitative PCR (RT-qPCR) analyses revealed that XopAM<sub>//Xcc// </sub> suppressed the expression of a WRKY transcription factor gene (WRKY33) associated with pattern-triggered immunity (Chen //et al.//, 2026).
 === Localization === === Localization ===
  
 XopAM<sub>Xcc</sub> is a lipase that may target the cytomembrane (Xie //et al.//, 2023). XopAM<sub>Xcc</sub> is a lipase that may target the cytomembrane (Xie //et al.//, 2023).
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 === Enzymatic function === === Enzymatic function ===
  
 XopAM<sub>Xcc</sub> is a lipase that may target the cytomembrane and this activity might be enhanced by its membrane-targeted protein XOPAM-ACTIVATED RESISTANCE 1 (AMAR1) in Arabidopsis Col-0 (Xie //et al.//, 2023). XopAM<sub>Xcc</sub> is a lipase that may target the cytomembrane and this activity might be enhanced by its membrane-targeted protein XOPAM-ACTIVATED RESISTANCE 1 (AMAR1) in Arabidopsis Col-0 (Xie //et al.//, 2023).
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 === Interaction partners === === Interaction partners ===
  
bacteria/t3e/xopam.txt · Last modified: 2026/10/29 15:09 by rkoebnik

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