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bacteria:t3e:xopal1 [2025/08/04 10:14] – [References] rkoebnikbacteria:t3e:xopal1 [2025/11/15 13:46] (current) – jfpothier
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 Author: [[https://www.researchgate.net/profile/Matthieu_Arlat|Matthieu Arlat]]\\ Author: [[https://www.researchgate.net/profile/Matthieu_Arlat|Matthieu Arlat]]\\
 Internal reviewer: [[https://www.researchgate.net/profile/Joana_Costa12|Joana Costa]], Anna Passelergue\\ Internal reviewer: [[https://www.researchgate.net/profile/Joana_Costa12|Joana Costa]], Anna Passelergue\\
-Expert reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]+Expert reviewer: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]\\
  
 Class: XopAL\\ Class: XopAL\\
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 Construction of a chimeric protein between the N-terminal region of XC_2995 (XopAL1) and a truncated AvrBS1 protein (AvrBS1<sub>59-445</sub>). The chimeric gene was introduced by conjugation into Xcc8004 ∆AvrBS1 mutant or derivative of this strain mutated in //hrpF// or //hpaB// genes. The transconjugants were tested for HR elicitation on pepper ECW-10R (Jiang //et al//., 2009). These experiments suggest that the N-terminal part of XopAL1 is able to allow the translocation of the chimeric protein into pepper cells in an Hrp-dependent manner. Construction of a chimeric protein between the N-terminal region of XC_2995 (XopAL1) and a truncated AvrBS1 protein (AvrBS1<sub>59-445</sub>). The chimeric gene was introduced by conjugation into Xcc8004 ∆AvrBS1 mutant or derivative of this strain mutated in //hrpF// or //hpaB// genes. The transconjugants were tested for HR elicitation on pepper ECW-10R (Jiang //et al//., 2009). These experiments suggest that the N-terminal part of XopAL1 is able to allow the translocation of the chimeric protein into pepper cells in an Hrp-dependent manner.
  
-The homolog in //X. translucens// pv. //translucens// strain DSM 18974 (BFP94_RS19935) was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).+The homolog in //X. translucens// pv. //translucens// strain DSM 18974 (BFP94_RS19935) was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013; Passelergue, 2025).
 === Regulation === === Regulation ===
  
bacteria/t3e/xopal1.1754298883.txt.gz · Last modified: 2025/08/04 10:14 by rkoebnik

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