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bacteria:t3e:xopal1 [2025/08/04 10:12] – [Biological function] rkoebnikbacteria:t3e:xopal1 [2025/08/04 10:15] (current) – [Biological function] rkoebnik
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 Construction of a chimeric protein between the N-terminal region of XC_2995 (XopAL1) and a truncated AvrBS1 protein (AvrBS1<sub>59-445</sub>). The chimeric gene was introduced by conjugation into Xcc8004 ∆AvrBS1 mutant or derivative of this strain mutated in //hrpF// or //hpaB// genes. The transconjugants were tested for HR elicitation on pepper ECW-10R (Jiang //et al//., 2009). These experiments suggest that the N-terminal part of XopAL1 is able to allow the translocation of the chimeric protein into pepper cells in an Hrp-dependent manner. Construction of a chimeric protein between the N-terminal region of XC_2995 (XopAL1) and a truncated AvrBS1 protein (AvrBS1<sub>59-445</sub>). The chimeric gene was introduced by conjugation into Xcc8004 ∆AvrBS1 mutant or derivative of this strain mutated in //hrpF// or //hpaB// genes. The transconjugants were tested for HR elicitation on pepper ECW-10R (Jiang //et al//., 2009). These experiments suggest that the N-terminal part of XopAL1 is able to allow the translocation of the chimeric protein into pepper cells in an Hrp-dependent manner.
  
-The homolog in //X. translucens// pv. //translucens// strain DSM 18974 (BFP94_RS19935) was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).+The homolog in //X. translucens// pv. //translucens// strain DSM 18974 (BFP94_RS19935) was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013; Passelergue, 2025).
 === Regulation === === Regulation ===
  
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 Jiang W, Jiang BL, Xu RQ, Huang JD, Wei HY, Jiang GF, Cen WJ, Liu J, Ge YY, Li GH, Su LL, Hang XH, Tang DJ, Lu GT, Feng JX, He YQ, Tang JL (2009). Identification of six type III effector genes with the PIP box in //Xanthomonas campestris// pv. //campestris// and five of them contribute individually to full pathogenicity. Mol. Plant Microbe Interact. 22: 1401-1411. DOI: [[https://doi.org/10.1094/MPMI-22-11-1401|10.1094/MPMI-22-11-1401]] Jiang W, Jiang BL, Xu RQ, Huang JD, Wei HY, Jiang GF, Cen WJ, Liu J, Ge YY, Li GH, Su LL, Hang XH, Tang DJ, Lu GT, Feng JX, He YQ, Tang JL (2009). Identification of six type III effector genes with the PIP box in //Xanthomonas campestris// pv. //campestris// and five of them contribute individually to full pathogenicity. Mol. Plant Microbe Interact. 22: 1401-1411. DOI: [[https://doi.org/10.1094/MPMI-22-11-1401|10.1094/MPMI-22-11-1401]]
 +
 +Koebnik R, Krüger A, Thieme F, Urban A, Bonas U (2006). Specific binding of the //Xanthomonas campestris// pv. //vesicatoria// AraC-type transcriptional activator HrpX to plant-inducible promoter boxes. J. Bacteriol. 188: 7652-7660. DOI: [[https://doi.org/10.1128/JB.00795-06|10.1128/JB.00795-06]]
  
 Nissinen RM, Ytterberg AJ, Bogdanove AJ, VAN Wijk KJ, Beer SV (2007). Analyses of the secretomes of //Erwinia amylovora// and selected hrp mutants reveal novel type III secreted proteins and an effect of HrpJ on extracellular harpin levels. Mol. Plant Pathol. 8: 55-67. DOI: [[https://doi.org/10.1111/j.1364-3703.2006.00370.x|10.1111/j.1364-3703.2006.00370.x]] Nissinen RM, Ytterberg AJ, Bogdanove AJ, VAN Wijk KJ, Beer SV (2007). Analyses of the secretomes of //Erwinia amylovora// and selected hrp mutants reveal novel type III secreted proteins and an effect of HrpJ on extracellular harpin levels. Mol. Plant Pathol. 8: 55-67. DOI: [[https://doi.org/10.1111/j.1364-3703.2006.00370.x|10.1111/j.1364-3703.2006.00370.x]]
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 Roux B, Bolot S, Guy E, Denancé N, Lautier M, Jardinaud MF, Fischer-Le Saux M, Portier P, Jacques MA, Gagnevin L, Pruvost O, Lauber E, Arlat M, Carrère S, Koebnik R, Noël LD (2015). Genomics and transcriptomics of //Xanthomonas campestris// species challenge the concept of core type III effectome. BMC Genomics 16: 975. DOI: [[https://doi.org/10.1186/s12864-015-2190-0|10.1186/s12864-015-2190-0]] Roux B, Bolot S, Guy E, Denancé N, Lautier M, Jardinaud MF, Fischer-Le Saux M, Portier P, Jacques MA, Gagnevin L, Pruvost O, Lauber E, Arlat M, Carrère S, Koebnik R, Noël LD (2015). Genomics and transcriptomics of //Xanthomonas campestris// species challenge the concept of core type III effectome. BMC Genomics 16: 975. DOI: [[https://doi.org/10.1186/s12864-015-2190-0|10.1186/s12864-015-2190-0]]
 +
 +Wengelnik K, Bonas U (1996). HrpXv, an AraC-type regulator, activates expression of five of the six loci in the hrp cluster of //Xanthomonas campestris// pv. //vesicatoria//. J. Bacteriol. 178: 3462-3469. DOI: [[https://doi.org/10.1128/jb.178.12.3462-3469.1996|10.1128/jb.178.12.3462-3469.1996]]
 +
 +Wengelnik K, Van den Ackerveken G, Bonas U (1996). HrpG, a key hrp regulatory protein of //Xanthomonas campestris// pv. //vesicatoria// is homologous to two-component response regulators. Mol. Plant Microbe Interact. 9: 704-712. DOI: [[https://doi.org/10.1094/mpmi-9-0704|10.1094/mpmi-9-0704]]
  
 Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]] Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]]
bacteria/t3e/xopal1.1754298723.txt.gz · Last modified: 2025/08/04 10:12 by rkoebnik

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