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bacteria:t3e:xopal1 [2025/02/12 23:34] – jfpothierbacteria:t3e:xopal1 [2025/08/01 13:02] (current) – apasselergue
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 ====== The Type III Effector XopAL1 from //Xanthomonas// ====== ====== The Type III Effector XopAL1 from //Xanthomonas// ======
  
-Author: [[https://www.researchgate.net/profile/Matthieu_Arlat|Matthieu Arlat]]\\ +Author: (XC_2995)[[https://www.researchgate.net/profile/Matthieu_Arlat|Matthieu Arlat]], (BFP94_RS19935)Anna Passelergue\\ 
-Internal reviewer: [[https://www.researchgate.net/profile/Joana_Costa12|Joana Costa]]+Internal reviewer: (XC_2995)[[https://www.researchgate.net/profile/Joana_Costa12|Joana Costa]], (BFP94_RS19935)[[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]
  
 Class: XopAL\\ Class: XopAL\\
 Family: XopAL1\\ Family: XopAL1\\
-Prototype: XC_2995/XopXccE1 (//Xanthomonas campestris// pv. //campestris//; strain 8004) (Jiang //et al.//, 2009)\\ +Prototype: XC_2995/XopXccE1 (//Xanthomonas campestris// pv. //campestris//; strain 8004) (Jiang //et al.//, 2009), BFP94_RS19935 (//Xanthomonas translucens// pv. //translucens//; strain DSM 18974)\\ 
-GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/AAY50043.1|AAY50043.1]] (332 aa)\\ +GenBank ID: (XC_2995)[[https://www.ncbi.nlm.nih.gov/protein/AAY50043.1|AAY50043.1]] (332 aa)\\ 
-RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_274360846.1|WP_274360846.1]] (305 aa)\\+RefSeq ID: (XC_2995)[[https://www.ncbi.nlm.nih.gov/protein/WP_274360846.1|WP_274360846.1]] (305 aa), (BFP94_RS19935)[[https://www.ncbi.nlm.nih.gov/protein/WP_003478560.1|WP_003478560.1]] (308 aa)\\ 
 +\\
 3D structure: Unknown 3D structure: Unknown
  
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 === How discovered? === === How discovered? ===
  
-XopAL was first identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate type III secreted (T3S)-effector due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_2995 (Jiang //et al.//, 2009).+XopAL (XC_2995) was first identified in //X. campestris// pv. //campestris// (//Xcc//) strain 8004 as a candidate type III secreted (T3S)-effector due to the presence of a plant-inducible promoter (PIP) box in its gene, XC_2995 (Jiang //et al.//, 2009). 
 + 
 +XopAL1 (BFP94_RS19935) was discovered as an ORF that is encoded downstream of a PIP box and a properly spaced ‐10 promoter motif (TTCGB‐N<sub>15</sub> ‐TTCGB‐N<sub>30–32</sub> ‐YANNNT) (Passelergue, 2025).
 === (Experimental) evidence for being a type III secreted effector (T3E) === === (Experimental) evidence for being a type III secreted effector (T3E) ===
  
 Construction of a chimeric protein between the N-terminal region of XC_2995 (XopAL1) and a truncated AvrBS1 protein (AvrBS1<sub>59-445</sub>). The chimeric gene was introduced by conjugation into Xcc8004 ∆AvrBS1 mutant or derivative of this strain mutated in //hrpF// or //hpaB// genes. The transconjugants were tested for HR elicitation on pepper ECW-10R (Jiang //et al//., 2009). These experiments suggest that the N-terminal part of XopAL1 is able to allow the translocation of the chimeric protein into pepper cells in an Hrp-dependent manner. Construction of a chimeric protein between the N-terminal region of XC_2995 (XopAL1) and a truncated AvrBS1 protein (AvrBS1<sub>59-445</sub>). The chimeric gene was introduced by conjugation into Xcc8004 ∆AvrBS1 mutant or derivative of this strain mutated in //hrpF// or //hpaB// genes. The transconjugants were tested for HR elicitation on pepper ECW-10R (Jiang //et al//., 2009). These experiments suggest that the N-terminal part of XopAL1 is able to allow the translocation of the chimeric protein into pepper cells in an Hrp-dependent manner.
 +
 +XopAL1 (BFP94_RS19935) was shown to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
 === Regulation === === Regulation ===
  
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 Yes, //X. campestris//, //X. translucens//, //X. arboricola// (Cesbron //et al//., 2015) Yes, //X. campestris//, //X. translucens//, //X. arboricola// (Cesbron //et al//., 2015)
 +
 +But also in //X. graminis// and //X. hortorum//.
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
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 Nissinen RM, Ytterberg AJ, Bogdanove AJ, VAN Wijk KJ, Beer SV (2007). Analyses of the secretomes of //Erwinia amylovora// and selected hrp mutants reveal novel type III secreted proteins and an effect of HrpJ on extracellular harpin levels. Mol. Plant Pathol. 8: 55-67. DOI: [[https://doi.org/10.1111/j.1364-3703.2006.00370.x|10.1111/j.1364-3703.2006.00370.x]] Nissinen RM, Ytterberg AJ, Bogdanove AJ, VAN Wijk KJ, Beer SV (2007). Analyses of the secretomes of //Erwinia amylovora// and selected hrp mutants reveal novel type III secreted proteins and an effect of HrpJ on extracellular harpin levels. Mol. Plant Pathol. 8: 55-67. DOI: [[https://doi.org/10.1111/j.1364-3703.2006.00370.x|10.1111/j.1364-3703.2006.00370.x]]
 +
 +Passelergue A (2025). Discovery of eight type III effector genes harboring the PIP box in clade-I xanthomonads. Master's thesis, Université de Montpellier, France.
  
 Peeters N, Carrère S, Anisimova M, Plener L, Cazalé AC, Genin S (2013). Repertoire, unified nomenclature and evolution of the Type III effector gene set in the //Ralstonia solanacearum// species complex. BMC Genomics 14: 859. DOI: [[https://doi.org/10.1186/1471-2164-14-859|10.1186/1471-2164-14-859]] Peeters N, Carrère S, Anisimova M, Plener L, Cazalé AC, Genin S (2013). Repertoire, unified nomenclature and evolution of the Type III effector gene set in the //Ralstonia solanacearum// species complex. BMC Genomics 14: 859. DOI: [[https://doi.org/10.1186/1471-2164-14-859|10.1186/1471-2164-14-859]]
  
 Roux B, Bolot S, Guy E, Denancé N, Lautier M, Jardinaud MF, Fischer-Le Saux M, Portier P, Jacques MA, Gagnevin L, Pruvost O, Lauber E, Arlat M, Carrère S, Koebnik R, Noël LD (2015). Genomics and transcriptomics of //Xanthomonas campestris// species challenge the concept of core type III effectome. BMC Genomics 16: 975. DOI: [[https://doi.org/10.1186/s12864-015-2190-0|10.1186/s12864-015-2190-0]] Roux B, Bolot S, Guy E, Denancé N, Lautier M, Jardinaud MF, Fischer-Le Saux M, Portier P, Jacques MA, Gagnevin L, Pruvost O, Lauber E, Arlat M, Carrère S, Koebnik R, Noël LD (2015). Genomics and transcriptomics of //Xanthomonas campestris// species challenge the concept of core type III effectome. BMC Genomics 16: 975. DOI: [[https://doi.org/10.1186/s12864-015-2190-0|10.1186/s12864-015-2190-0]]
 +
 +Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]]
  
 ===== Acknowledgements ===== ===== Acknowledgements =====
bacteria/t3e/xopal1.1739403251.txt.gz · Last modified: 2025/02/12 23:34 by jfpothier

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