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bacteria:t3e:xopah [2020/07/09 11:58] – [References] rkoebnikbacteria:t3e:xopah [2025/02/21 11:35] (current) – [Conservation] rkoebnik
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-====== XopAH ======+====== The Type III Effector XopAH from //Xanthomonas// ======
  
 Author: [[https://www.researchgate.net/profile/Steven_Roberts8|Steven J. Roberts]]\\ Author: [[https://www.researchgate.net/profile/Steven_Roberts8|Steven J. Roberts]]\\
-Internal reviewer: [[https://www.researchgate.net/profile/Christian_Verniere|Christian Vernière ]]\\ +Internal reviewer: [[https://www.researchgate.net/profile/Christian_Verniere|Christian Vernière ]]
-Expert reviewer: FIXME+
  
 Class: XopAH\\ Class: XopAH\\
 Family: XopAH\\ Family: XopAH\\
-Prototype: AvrXccC (//Xanthomonas campestris// pv. //campestris//; strain 8004) (Qian //et al//., 2005)\\ +Prototype: AvrXccC (//Xanthomonas campestris// pv. //campestris//; strain 8004)\\ 
-RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/ABQ10636.1|ABQ10636.1]] (440 aa) (gene [[https://www.ncbi.nlm.nih.gov/nuccore/EF529437.1|EF529437.1]] 1323 bp)\\ +GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/ABQ10636.1|ABQ10636.1]] (440 aa)\\ 
-Synonym: AvrXccC (//Xanthomonas campestris// pv. //campestris//)\\+RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011037263.1|WP_011037263.1]] (331 aa, perhaps 109 aa too short since the 440-aa version would include a typical N-terminal palmitoylation signal, MGLC)\\ 
 +Synonym: AvrXccC (//Xanthomonas campestris// pv. //campestris//), AvrXccFM (//Xanthomonas campestris// pv. //campestris//) (Castañeda //et al.//, 2005)\\
 3D structure: Unknown 3D structure: Unknown
  
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 === (Experimental) evidence for being a T3E === === (Experimental) evidence for being a T3E ===
  
-Secreted XopAH (AvrXccC) proteins were detected in culture fluid from //Xcc// 8004 and //hrcV// mutant complemented strains but not from the //hrcV// mutant (Wang //et al//., 2007). Insertion and deletion mutants affecting the locus (Xcc2109) in the type strain (Xcc 528) resulted in loss of virulence on the host Florida Broad Leaf Mustard (Castenada //et al//., 2005).+Secreted XopAH (AvrXccC) proteins were detected in culture fluid from //Xcc// 8004 and //hrcV// mutant complemented strains but not from the //hrcV// mutant (Wang //et al//., 2007). Insertion and deletion mutants affecting the locus (Xcc2109) in the type strain (Xcc 528) resulted in loss of virulence on the host Florida Broad Leaf Mustard (Castañeda //et al//., 2005).
 === Regulation === === Regulation ===
  
-Promoter activity assays showed that the expression of XopAH (//avrXccC)// is //hrpG/hrpX//-dependent (Wang //et al//., 2007).+Promoter activity assays showed that the expression of XopAH (//avrXccC//) is //hrpG/hrpX//-dependent (Wang //et al//., 2007).
 === Phenotypes === === Phenotypes ===
  
-This effector is required for full virulence in the susceptible host cabbage (//Brassica oleracea//) (Wang //et al//., 2007) and results in avirulence in the resistant host mustard (//Brassica napiformis//) (Castenada //et al//., 2005; He //et al//., 2007; Wang //et al//., 2007). The intact AvrB-AvrC domain of //AvrXccC<sub>8004</sub> // is essential and sufficient to elicit defense responses in an //Arabidopsis// resistant ecotype (Col-0) (Ho et al., 2013).+This effector is required for full virulence in the susceptible host cabbage (//Brassica oleracea//) (Wang //et al//., 2007) and results in avirulence in the resistant host mustard (//Brassica napiformis//) (Castaneda //et al//., 2005; He //et al//., 2007; Wang //et al//., 2007). The intact AvrB-AvrC domain of //AvrXccC<sub>8004</sub> // is essential and sufficient to elicit defense responses in an //Arabidopsis// resistant ecotype (Col-0) (Ho //et al//., 2013).
  
-In the interaction //Arabidopsis// / //Xcc// strain 8004, //AvrXccC<sub>8004</sub> // not only presented its avirulence activity to trigger plant defense response but also possessed its virulence activity to manipulate the component involved in the ABA signalling pathway leading to an increase of ABA concentrations (Ho //et al//., 2013).+In the interaction //Arabidopsis// / //Xcc// strain 8004, AvrXccC<sub>8004</sub> not only presented its avirulence activity to trigger plant defense response but also possessed its virulence activity to manipulate the component involved in the ABA signalling pathway leading to an increase of ABA concentrations (Ho //et al.//, 2013).
 === Localization === === Localization ===
  
-XopAH (AvrXccC) is anchored to the plant plasma membrane, and the N‐terminal myristoylation site (amino acids 2–7: GLcaSK) is essential for its localization (Wang //et al//., 2007).+XopAH (AvrXccC) is anchored to the plant plasma membrane, and the N‐terminal myristoylation site (amino acids 2–7: GLcaSK) is essential for its localization (Wang //et al.//, 2007).
 === Enzymatic function === === Enzymatic function ===
  
-XopAH has a Fido/AvrB domain derived from the fic (cyclic adenosine monophosphate (cAMP)-induced filamentation and doc (death on curing) domains (Kinch //et al//., 2009). Structural comparisons resulted in the inclusion of similar segments of the T3 effector AvrB from //Pseudomonas syringae// species (Kinch //et al//., 2009; White //et al//., 2009). T3 effectors in the XopAH group could trans-AMPylate plant host proteins. AMPylation represents a posttranslational modification used to stably modify proteins with AMP (Kinch //et al//., 2009).+XopAH has a Fido/AvrB domain derived from the fic (cyclic adenosine monophosphate (cAMP)-induced filamentation and doc (death on curing) domains (Kinch //et al//., 2009). Structural comparisons resulted in the inclusion of similar segments of the T3 effector AvrB from //Pseudomonas syringae// species (Kinch //et al//., 2009; White //et al//., 2009). T3 effectors in the XopAH group could trans-AMPylate plant host proteins. AMPylation represents a posttranslational modification used to stably modify proteins with AMP (Kinch //et al.//, 2009).
 === Interaction partners === === Interaction partners ===
  
-Not known ?+Not known.
  
 ===== Conservation ===== ===== Conservation =====
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 === In xanthomonads === === In xanthomonads ===
  
-In //Xanthomonas campestris// pv. campestris. XopAH is also present in //X. arboricola// pv. juglandis within strains causing Walnut Blight but is absent from the strains causing vertical oozing canker (Cesbron et al., 2015).+In //Xanthomonas campestris// pv. campestris. XopAH is also present in //X. arboricola// pv. juglandis within strains causing Walnut Blight but is absent from the strains causing vertical oozing canker (Cesbron //et al.//, 2015).
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
 Yes (AvrB //Pseudomonas savastanoi//, //Pseudomonas syringae//) (Lee //et al.//, 2004; Desveaux //et al.//, 2007) Yes (AvrB //Pseudomonas savastanoi//, //Pseudomonas syringae//) (Lee //et al.//, 2004; Desveaux //et al.//, 2007)
 +
 ===== References ===== ===== References =====
  
-Castenada A, Reddy JD, El-Yacoubi B, Gabriel DW (2005). Mutagenesis of all eight avr genes in //Xanthomonas campestris// pv. //campestris// had no detected effect on pathogenicity, but one //avr// gene affected race specificity. Mol. Plant Microbe Interact. 18: 1306-1317. DOI: [[https://doi.org/10.1094/MPMI-18-1306|10.1094/MPMI-18-1306]]+Castañeda A, Reddy JD, El-Yacoubi B, Gabriel DW (2005). Mutagenesis of all eight avr genes in //Xanthomonas campestris// pv. //campestris// had no detected effect on pathogenicity, but one //avr// gene affected race specificity. Mol. Plant Microbe Interact. 18: 1306-1317. DOI: [[https://doi.org/10.1094/MPMI-18-1306|10.1094/MPMI-18-1306]]
  
 Cesbron S, Briand M, Essakhi S, Gironde S, Boureau T, Manceau C, Fischer-Le Saux M, Jacques MA (2015). Comparative genomics of pathogenic and nonpathogenic strains of //Xanthomonas arboricola// unveil molecular and evolutionary events linked to pathoadaptation. Front. Plant Sci. 6: 1126. DOI: [[https://doi.org/10.3389/fpls.2015.01126|10.3389/fpls.2015.01126]] Cesbron S, Briand M, Essakhi S, Gironde S, Boureau T, Manceau C, Fischer-Le Saux M, Jacques MA (2015). Comparative genomics of pathogenic and nonpathogenic strains of //Xanthomonas arboricola// unveil molecular and evolutionary events linked to pathoadaptation. Front. Plant Sci. 6: 1126. DOI: [[https://doi.org/10.3389/fpls.2015.01126|10.3389/fpls.2015.01126]]
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 White FF, Potnis N, Jones JB, Koebnik R (2009). The type III effectors of //Xanthomonas//. Mol. Plant Pathol. 10: 749-766. DOI: [[https://doi.org/10.1111/j.1364-3703.2009.00590.x|10.1111/j.1364-3703.2009.00590.x]] White FF, Potnis N, Jones JB, Koebnik R (2009). The type III effectors of //Xanthomonas//. Mol. Plant Pathol. 10: 749-766. DOI: [[https://doi.org/10.1111/j.1364-3703.2009.00590.x|10.1111/j.1364-3703.2009.00590.x]]
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
  
bacteria/t3e/xopah.1594292319.txt.gz · Last modified: 2023/01/09 10:20 (external edit)

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