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--- bacteria:t3e:xopaf [2020/07/01 08:51] – [References] rkoebnik
+++ bacteria:t3e:xopaf [2025/02/12 23:16] (current) – jfpothier
@@ Line 1: / Line 1: @@
-====== XopAF ======
+====== The Type III Effector XopAF from //Xanthomonas// ======
-Author: Tamas Kovacs\\
+Author: [[https://www.researchgate.net/profile/Tamas_Kovacs6|Tamás Kovács]]\\
-Internal reviewer: FIXME\\
+Internal reviewer: [[https://www.researchgate.net/profile/Guido_Sessa|Guido Sessa]]
-Expert reviewer: FIXME
 Class: XopAF\\
-Family: XopAF\\
+Families: XopAF1, XopAF2\\
-Prototype: XopAF (//Xanthomonas campestris// pv. vesicatoria tomato race 3)\\
+Prototype: AvrXv3 (//Xanthomonas euvesicatoria// pv. //perforans//; strain T3)\\
-RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/|WP_145590005.1]] (218 aa)\\
+GenBank ID (XopAF1): [[https://www.ncbi.nlm.nih.gov/protein/AAG18480.1|AAG18480.1]] (218 aa)\\
+RefSeq ID (XopAF1): [[https://www.ncbi.nlm.nih.gov/protein/WP_008577605.1|WP_008577605.1]] (218 aa)\\
+RefSeq ID (XopAF2): [[https://www.ncbi.nlm.nih.gov/protein/WP_227479740.1|WP_227479740.1]] (286 aa)\\
+Synonym: AvrXv3\\
 D structure: Unknown
@@ Line 14: / Line 16: @@
 === How discovered? ===
-AvrXv3 was discovered by genetic screen and cloning and sequencing its encoding gene from //Xanthomonas campestris// pv. vesicatoria (Minsavage //et al//., 1996).
+AvrXv3 was discovered by genetic screen and cloning and sequencing its encoding gene from //Xanthomonas euvesicatoria// pv. perforans (Minsavage //et al//., 1996).
 === (Experimental) evidence for being a T3E ===
 Signal sequence analysis (Roden //et al//., 2004).
 === Regulation ===
-Induced; PIP box (Astua-Monge //et al//., 2000).
+Induced; PIP box (Astua-Monge //et al//., 2000).
 === Phenotypes ===
-Expression studies with a fusion of this gene and //uidA// indicated that avrXv3 is plant inducible and controlled by the hypersensitivity and pathogenicity (hrp) regulatory system. Mutational analysis and transcription activation assays revealed that AvrXv3 has transcription activation activity in yeast, and that the putative domain responsible for that activity is located at the C terminus of the AvrXv3 protein. //Agrobacterium tumefaciens//-mediated transient expression confirmed the direct role of AvrXv3 in eliciting the HR in tomato NIL 216 and supported the hypothesis that Avr proteins must be present inside the plant host cell to trigger the HR (Astua-Monge //et al//., 2000).
+Expression studies with a fusion of this gene and //uidA// indicated that avrXv3 is plant inducible and controlled by the hypersensitivity and pathogenicity (hrp) regulatory system. Mutational analysis and transcription activation assays revealed that AvrXv3 has transcription activation activity in yeast, and that the putative domain responsible for that activity is located at the C terminus of the AvrXv3 protein. //Agrobacterium tumefaciens//-mediated transient expression confirmed the direct role of AvrXv3 in eliciting the hypersensitive response (HR) in tomato NIL 216 and supported the hypothesis that Avr proteins must be present inside the plant host cell to trigger the HR (Astua-Monge //et al//., 2000). Tomato genes differential expressed during the resistance response triggered by AvrXv3 recognition were identified by microarray analysis (Balaji et al., 2007).
 === Localization ===
 Inside the plant host cell, no further details are available.
 === Enzymatic function ===
-Unknown. The orthologue HopAF1 is a deaminase, inhibits ethylene biosynthesis (Washington //et al//., 2016).
+Unknown. The orthologue HopAF1 is a deaminase, inhibits ethylene biosynthesis (Washington //et al//., 2016).
 === Interaction partners ===
-RxvT3 in tomato (has not been identified yet).
+Tomato RxvT3 resistance protein (has not been identified yet).
 ===== Conservation =====
 === In xanthomonads ===
-Yes (Washington //et al//., 2016).
+Yes (//e.g.//, //X. alfalfae//, //X. citri//, //X. translucens//) (Washington //et al//., 2016).
 === In other plant pathogens/symbionts ===
-Yes (Washington //et al//., 2016).
+Yes (//Pseudomonas, Acidovorax//, //Ralstonia// spp.) (Washington //et al//., 2016).
 ===== References =====
-Astua-Monge G, Minsavage VG, Stall ER, Davis JM, Bonas U, Jones BJ (2000). Resistance of tomato and pepper to T3 strains of //Xanthomonas campestris// pv. vesicatoria is specified by a plant-inducible avirulence gene. Mol. Plant Microbe Interact. 13: 911-921. DOI: [[https://doi.org/10.1094/MPMI.2000.13.9.911|10.1094/MPMI.2000.13.9.911]]
+Astua-Monge G, Minsavage VG, Stall ER, Davis JM, Bonas U, Jones BJ (2000). Resistance of tomato and pepper to T3 strains of //Xanthomonas campestris// pv. vesicatoria is specified by a plant-inducible avirulence gene. Mol. Plant Microbe Interact. 13: 911-921. DOI: [[http://doi.org10.1007/s10142-007-0050-y|10.1094/MPMI.2000.13.9.911]]
-Minsavage GV, Jones JB, Stall RE (1996). Cloning and sequencing of an avirulence gene (//avrRxv3//) isolated from //Xanthomonas campestris// pv. vesicatoria tomato race 3. Phytopathology 86: S15. DOI: [[https://doi.org/10.1094/mpmi-6-616|10.1094/mpmi-6-616]]
+Balaji V, Gibly A, Debbie P, Sessa G (2007). Transcriptional analysis of the tomato resistance response triggered by recognition of the //Xanthomonas// type III effector AvrXv3. Funct. Integr. Genomics 7: 305-3016. DOI: [[https://doi.org/10.1007/s10142-007-0050-y|https://doi.org/10.1007/s10142-007-0050-y]]
+Minsavage GV, Jones JB, Stall RE (1996). Cloning and sequencing of an avirulence gene (//avrRxv3//) isolated from //Xanthomonas campestris// pv. vesicatoria tomato race 3. Phytopathology 86: S15.
 Roden AJ, Belt B, Ross BJ, Tachibana T, Vargas J, Mudgett BM (2004). A genetic screen to isolate type III effectors traslocated into pepper cells during //Xanthomonas// infection. Proc. Natl. Acad. Sci. USA 101: 16624-16629. DOI: [[https://doi.org/10.1073/pnas.0407383101|10.1073/pnas.0407383101]]
 Washington EJ, Mukhtar MS, Finkel MO, Wan L, Banfield JM, Kieber JJ, Dangl LJ (2016). //Pseudomonas syringae// type III effector HopAF1 suppresses plant immunity by targeting methionine recycling to block ethylene induction. Proc. Natl. Acad. Sci. USA. 113: E3577-E3586. DOI: [[https://doi.org/10.1073/pnas.1606322113|10.1073/pnas.1606322113]]
+===== Further reading =====
+Gibly A, Bonshtien A, Balaji V, Debbie P, Martin GB, Sessa G (2004). Identification and expression profiling of tomato genes differentially regulated during a resistance response to //Xanthomonas campestris// pv. //vesicatoria//. Mol. Plant Microbe Interact. 17: 1212-1222. DOI: [[https://doi.org/10.1094/MPMI.2004.17.11.1212|10.1094/MPMI.2004.17.11.1212]]
+Jalan N, Kumar D, Andrade MO, Yu F, Jones JB, Graham JH, White FF, Setubal JC, Wang N (2013). Comparative genomic and transcriptome analyses of pathotypes of //Xanthomonas citri// subsp. //citri// provide insights into mechanisms of bacterial virulence and host range. BMC Genomics 14: 551. DOI: [[https://doi.org/10.1186/1471-2164-14-551|10.1186/1471-2164-14-551]]
+Timilsina S, Abrahamian P, Potnis N, Minsavage GV, White FF, Staskawicz BJ, Jones JB, Vallad GE, Goss EM (2016). Analysis of sequenced genomes of //Xanthomonas perforans// identifies candidate targets for resistance breeding in tomato. Phytopathology 106: 1097-1104. DOI: [[https://doi.org/10.1094/PHYTO-03-16-0119-FI|10.1094/PHYTO-03-16-0119-FI]]
+===== Acknowledgements =====
+This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).

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