EuroXanth DokuWiki

User Tools

Site Tools

Trace:
bacteria:t3e:xopaa

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revisionPrevious revision
Next revision		Previous revision
bacteria:t3e:xopaa [2023/12/08 12:21] – [Biological function] rkoebnikbacteria:t3e:xopaa [2025/07/13 23:57] (current) – jfpothier
Line 1: Line 1:
-====== XopAA ======+====== The Type III Effector XopAA from //Xanthomonas// ======
  
 Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\ Author: [[https://www.researchgate.net/profile/Ralf-Koebnik|Ralf Koebnik]]\\
-Internal reviewer:\\ 
-Expert reviewer: **WANTED!** 
  
 Class: XopAA\\ Class: XopAA\\
Line 25: Line 23:
  
 //Xanthomonas// bacteria carrying a translational fusion of XopAA to the calmodulin-dependent adenylate cyclase Cya produced approximately 30 times more cAMP in tomato leaves at 8 hpi when compared to bacteria with a mutation in the T3SS gene //hrcV// or bacteria that harbour a //recA//://cya// fusion. These results indicated that the //xopAA// gene product is translocated to host cells in a T3SS-dependent manner (Morales //et al.//, 2005). //Xanthomonas// bacteria carrying a translational fusion of XopAA to the calmodulin-dependent adenylate cyclase Cya produced approximately 30 times more cAMP in tomato leaves at 8 hpi when compared to bacteria with a mutation in the T3SS gene //hrcV// or bacteria that harbour a //recA//://cya// fusion. These results indicated that the //xopAA// gene product is translocated to host cells in a T3SS-dependent manner (Morales //et al.//, 2005).
 +
 === Regulation === === Regulation ===
  
-RT-PCR expression analyses suggested that, although translocation of XopAA is dependent on the Hrp T3SS apparatus, //xopAA// expression does not depend on nutritional conditions that induce //hrp// gene expression in //Xee // (Morales //et al.//, 2005).+RT-PCR expression analyses suggested that, although translocation of XopAA is dependent on the Hrp T3SS apparatus, //xopAA// expression does not depend on nutritional conditions that induce //hrp// gene expression in //Xee// (Morales //et al.//, 2005). 
 === Phenotypes === === Phenotypes ===
  
-Transgenic rice plants expressing XopAA//<sub>Xoo</sub> // exhibited semi-dwarfism and a reduction in Brassinolide-dependent laminar inclination, characteristics of brassinosteroid (BR)-insensitive mutants caused by mutations of the BR receptor (Yamaguchi //et al.//, 2013).+Transgenic rice plants expressing XopAA//<sub>Xoo</sub>// exhibited semi-dwarfism and a reduction in Brassinolide-dependent laminar inclination, characteristics of brassinosteroid (BR)-insensitive mutants caused by mutations of the BR receptor (Yamaguchi //et al.//, 2013).
  
-Rice plants inoculated with a T3SS-deficient //hrpX// mutant of //X. oryzae// pv. //oryzae// (//Xoo//) did not develop disease lesions. In contrast, a transgenic rice line overexpressing //xopAA// showed severe lesions when inoculated with the //Xoo// //hrpX// mutant and the bacterial population of the //hrpX// mutant was 100-fold higher in the transgenic plants than in wild-type plants. This observation led the authors conclude that XopAA<sub>//Xoo// </sub> inhibits resistance to //Xoo//, which was probably caused by suppression of host PTI (Yamaguchi //et al.//, 2013). However, a //xopAA// knock-out strain of //Xoo //did not exhibit any defect in virulence (Yamaguchi //et al.//, 2013).+Rice plants inoculated with a T3SS-deficient //hrpX// mutant of //X. oryzae// pv. //oryzae// (//Xoo//) did not develop disease lesions. In contrast, a transgenic rice line overexpressing //xopAA// showed severe lesions when inoculated with the //Xoo// //hrpX// mutant and the bacterial population of the //hrpX// mutant was 100-fold higher in the transgenic plants than in wild-type plants. This observation led the authors conclude that XopAA<sub>//Xoo//</sub> inhibits resistance to //Xoo//, which was probably caused by suppression of host PTI (Yamaguchi //et al.//, 2013). However, a //xopAA// knock-out strain of //Xoo// did not exhibit any defect in virulence (Yamaguchi //et al.//, 2013).
  
-Expression of XopAA//<sub>Xoo</sub> // in //Arabidopsis// cells activated host immune responses, suggesting the presence of intracellular immune receptors that recognize XopAA (Yamaguchi //et al.//, 2013).+Expression of XopAA<sub>//Xoo//</sub> in //Arabidopsis// cells activated host immune responses, suggesting the presence of intracellular immune receptors that recognize XopAA (Yamaguchi //et al.//, 2013). 
 + 
 +//Xanthomonas oryzae// pv. //oryzicola// bacteria with a deletion of //xopAA// were found to cause enhanced virulence to a certain rice cultivar (Li //et al.//, 2015).
  
-//Xanthomonas oryzae //pv. //oryzicola// bacteria with a deletion of //xopAA// were found to cause enhanced virulence to a certain rice cultivar (Li //et al.//, 2015). 
 === Localization === === Localization ===
  
Line 47: Line 48:
 === Interaction partners === === Interaction partners ===
  
-A yeast two-hybrid experiment indicated that XopAA<sub>//Xoo// </sub> interacted with OsBAK1, an essential component of both microbe-associated molecular patterns (MAMPs) and BR receptors, suggesting that the virulent activity of XopAA is mediated by inhibition of OsBAK1 (Yamaguchi //et al.//, 2013).+A yeast two-hybrid experiment indicated that XopAA<sub>//Xoo//</sub> interacted with OsBAK1, an essential component of both microbe-associated molecular patterns (MAMPs) and BR receptors, suggesting that the virulent activity of XopAA is mediated by inhibition of OsBAK1 (Yamaguchi //et al.//, 2013).
  
 ===== Conservation ===== ===== Conservation =====
Line 54: Line 55:
  
 DNA hybridization experiments had suggested that //xopAA// might be highly specific to //Xee// (Morales //et al//., 2005). However, genomic comparisons showed that xopAA homologs are present in other xanthomonads, such as //X. hortorum//, //X. hydrangeae//, //X. oryzae//, //X. populi//, //X. translucens//, and //X. vasicola//. DNA hybridization experiments had suggested that //xopAA// might be highly specific to //Xee// (Morales //et al//., 2005). However, genomic comparisons showed that xopAA homologs are present in other xanthomonads, such as //X. hortorum//, //X. hydrangeae//, //X. oryzae//, //X. populi//, //X. translucens//, and //X. vasicola//.
 +
 === In other plant pathogens/symbionts === === In other plant pathogens/symbionts ===
  
Line 65: Line 67:
  
 Yamaguchi K, Nakamura Y, Ishikawa K, Yoshimura Y, Tsuge S, Kawasaki T (2013). Suppression of rice immunity by //Xanthomonas oryzae// type III effector Xoo2875. Biosci. Biotechnol. Biochem. 77: 796-801. DOI: [[https://doi.org/10.1271/bbb.120929|10.1271/bbb.120929]] Yamaguchi K, Nakamura Y, Ishikawa K, Yoshimura Y, Tsuge S, Kawasaki T (2013). Suppression of rice immunity by //Xanthomonas oryzae// type III effector Xoo2875. Biosci. Biotechnol. Biochem. 77: 796-801. DOI: [[https://doi.org/10.1271/bbb.120929|10.1271/bbb.120929]]
 +
 +===== Acknowledgements =====
 +
 +This fact sheet is based upon work from COST Action CA16107 EuroXanth, supported by COST (European Cooperation in Science and Technology).
 +
  
bacteria/t3e/xopaa.1702038085.txt.gz · Last modified: 2023/12/08 12:21 by rkoebnik

Page Tools

Except where otherwise noted, content on this wiki is licensed under the following license: CC Attribution-Share Alike 4.0 International
CC Attribution-Share Alike 4.0 International Donate Powered by PHP Valid HTML5 Valid CSS Driven by DokuWiki