The Type III Effector XopAV from //Xanthomonas//

Author: Ralf Koebnik

Class: XopAV
Families: XopAV1, XopAV2
Prototypes: XCV1197/XCV1198 (Xanthomonas euvesicatoria pv. euvesicatoria, ex Xanthomonas campestris pv. vescicatoria; strain 85-10) (Teper et al., 2016) and XC_3176 (Xanthomonas campestris pv. campestris; strain 8004) (Yang et al., 2015)
GenBank ID (XCV1197, probably including an N-terminal fragment of XopAV): CAJ22828.1 (165 aa)
GenBank ID (XCV1198, probably including a C-terminal fragment of XopAV): CAJ22829.1 (129 aa)
GenBank ID (XopAV2/XC_3176): AAY50220.1 (244 aa)
RefSeq ID (XopAV1): WP_065628638.1 (258 aa)
RefSeq ID (XopAV2): WP_011269951.1 (249 aa)
3D structure: Unknown

XopAV_Xcv85-10 (XCV1197) was discovered using a machine-learning approach (Teper et al., 2016).

XopAV_Xcv85-10 (XCV1197) fused to the AvrBs2 reporter was shown to translocate into plant cells in an hrpF-dependent manner (Teper et al., 2016). Similarly, when the promoter and signal region of XopAV_Xcc8004 (XC_3176) were fused to the HR-inducing AvrBs1 C-terminal domain lack of 58 N-terminal amino acid residues, this construct elicited an hypersensitive response on the pepper ECW-10R containing the corresponding resistance gene Bs1 in an hrcV-dependent manner (Yang et al., 2015).

A reporter GUS fusion of XopAV_Xcc8004 (XC_3176) displayed GUS activities, which were significantly lower in the mutant strains ΔhrpX and ΔhrpG than that in the wild type X. campestris pv. campestris strain (Yang et al., 2015).

XopAV_Xcc8004 (XC_3176) was found to be required for the full virulence of X. campestris pv. campestris strain 8004 when assayed on Chinese radish by the leaf-clipping method (Yang et al., 2015).

Unknown

Unknown

Unknown

Yes (e.g., X. arboricola, X. campestris, X. euvesicatoria, X. fragariae, X. hortorum, X. translucens)

No

Teper D, Burstein D, Salomon D, Gershovitz M, Pupko T, Sessa G (2016). Identification of novel Xanthomonas euvesicatoria type III effector proteins by a machine-learning approach. Mol. Plant Pathol. 17: 398-411. DOI: 10.1111/mpp.12288

Yang L, Su H, Yang F, Jian H, Zhou M, Jiang W, Jiang B (2015). Identification of a new type III effector XC3176 in Xanthomonas campestris pv. campestris. Acta Microbiologica Sinica 10: 1264-1272 / Wei Sheng Wu Xue Bao 55: 1264-1272. PubMed ID: 26939454. {Article in Chinese}