====== The Type III Effector XopAB from //Xanthomonas// ======

Author: [[https://www.researchgate.net/profile/Ralf_Koebnik|Ralf Koebnik]]

Class: XopAB\\
Family: XopAB\\
Prototype: XOO3150 (//Xanthomonas oryzae// pv. //oryzae//; strain T7174)\\
GenBank ID: [[https://www.ncbi.nlm.nih.gov/protein/BAE69905.1|BAE69905.1]] (193 aa)\\
RefSeq ID: [[https://www.ncbi.nlm.nih.gov/protein/WP_011409110.1| WP_011409110.1]] (193 aa)\\
3D structure: Unknown

===== Biological function =====

=== How discovered? ===

XopAB was discovered via genome-wide screening for effector candidates in //X. oryzae// pv. //oryzae// (//Xoo//) strain MAFF 311018 in accordance with three criteria: i) ORF encoding proteins homologous to effectors of //P. syringae// strains and //X. campestris// pv. //vesicatoria//, ii) HrpX regulons preceded by two cis elements (Plant Induced Promoter, PIP; and -10 box-like motif) or iii) proteins with an N-terminal amino acid composition similar to known effectors (more than 10% of Ser in the first 50 amino aa, no Asp or Glu residues in the first 12 aa, and an aliphatic amino acid or Pro at the third or fourth position) (Furutani //et al.//, 2009).
=== (Experimental) evidence for being a T3E ===

TT3S-dependent translocation of XopAB has been shown by a calmodulin-dependent adenylate cyclase reporter assay (Furutani //et al.//, 2009). XopR<sub>//Xoo// </sub> was confirmed to have a functional type III secretion signal using a reporter fusion with AvrBs1 (Zhao //et al.//, 2013).
=== Regulation ===

=== Phenotypes ===

=== Localization ===

=== Enzymatic function ===

=== Interaction partners ===

===== Conservation =====

=== In xanthomonads ===

=== In other plant pathogens/symbionts ===

===== References =====

Furutani A, Takaoka M, Sanada H, Noguchi Y, Oku T, Tsuno K, Ochiai H, Tsuge S (2009). Identification of novel type III secretion effectors in //Xanthomonas oryzae// pv. //oryzae.// Mol. Plant Microbe Interact. 22: 96-106. DOI: [[https://doi.org/10.1094/MPMI-22-1-0096|10.1094/MPMI-22-1-0096]]

Zhao S, Mo WL, Wu F, Tang W, Tang JL, Szurek B, Verdier V, Koebnik R, Feng JX (2013). Identification of non-TAL effectors in //Xanthomonas oryzae// pv. //oryzae// Chinese strain 13751 and analysis of their role in the bacterial virulence. World J. Microbiol. Biotechnol. 29: 733-744. DOI: [[https://doi.org/10.1007/s11274-012-1229-5|10.1007/s11274-012-1229-5]]